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Test Saliva at Salimetrics SalivaLab

Attributes of Our High-Quality Salivary Bioscience Laboratory

#1   Routine calibration of lab equipment

High-quality labs have all of their lab equipment in a scheduled, routine calibration program. Calibration of lab equipment allows for reproducible measurements; conversely, uncalibrated equipment is one of the principle sources of lab error and almost always guarantees that the same samples tested across different labs will have a high inter-lab assay variability.

#2   Rigorous quality acceptance criteria for assay results

A good assay manufacturer will provide assays that meet rigorous quality acceptance criteria, and then provide protocols and guidance that ensures each laboratory can achieve great results. It’s a simple fact: the tighter the acceptance criteria, the less variability in results; the wider the acceptance criteria, the greater the variability in results. Salimetrics’ assays are manufactured and quality tested to rigorous acceptance criteria, and the Salimetrics SalivaLab reliably achieves these standards.

Salivary Cortisol Standard Curves Overlay

#3   Documented CV% acceptance/repeat criteria

High-quality labs can control for many possible sources of lab error, but saliva samples are unique in that they may be viscous and heterogenous, and, if the saliva samples are not processed by the lab correctly, duplicate measurements of the same sample can have large deviations between the two measures. Consider duplicate measurements of the same sample having a CV% of 20%; this means that the two measurements are actually 40% apart, and the mean value is 20% apart from each of the two values. More often than not, only one of these measures is incorrect, so choosing the mean value suggests that the value intrinsically has a 20% error. Further, if the average CV% of the entire data set is 20%, then any conclusions made by the researcher would be unsupported if the conclusion did not rely on a >20% difference between the means.

Salivary Cortisol Precision Graph

In addition to following good protocols, a high-quality lab will avoid sample processing and handling errors by having well-documented criteria that only permits the acceptance of the duplicate mean based on a maximum allowable percentage of coefficient of variation between duplicate values, or when the absolute difference of the duplicate values is small and does not have biological significance. When the criteria are not met on a sample, the sample is rerun or excluded from the final data analysis. In addition, for quality control, the lab should record the sample as having been repeated.

 

 

#4   Laboratory personnel trained in salivary testing to run the assays

Understanding how to work with viscous samples to avoid the introduction of bubbles and tiny volume discrepancies when pipetting, how to prevent bias across the plate, and even how to read and interpret the aforementioned quality control data, can make a difference in the integrity of the results. Both inter- and intra-assay CVs are improved when adequately trained personnel run assays. A high-quality lab will incorporate exercises such as pipetting proficiency into the training of personnel, and document the results, before actual assays are run.

Saliva Pipetting Proficiency

*Note: Operators #2, 3 and 5 should be retrained, focusing on small volume pipetting techniques.

#5   Routinely processes saliva samples and performs saliva testing

Practice makes perfect. The Salimetrics SalivaLab has more expertise and experience at running assays compared to labs that run assays infrequently. It’s not that most labs are unable to get acceptable results from some of their salivary testing. It’s that most labs do not know how to get the best results from all of their saliva testing, and that’s simply because they have not tackled the full breadth of issues a proven, high-quality lab has experienced. Salimetrics’ lab knows how to troubleshoot and determine where potential issues may lie, and how to correct to get the best results.

#6   Participates in a proficiency testing program

Participating in a proficiency testing program is like getting a graded report card for the lab. It’s conducted after the lab meets all of the basic requirements for rigor, including training, and thereafter at minimum on an annual basis. For diagnostic labs, it is a regulatory requirement that they demonstrate proficiency in sample handling and processing by providing sample measurements on unknown, “controlled” samples. While this might not seem relevant to research, it is! A key finding from proficiency testing is that researchers can trust that the results because the results are repeatable.

#7   Uses and recommends validated saliva collection methods

High-quality labs know that how a sample is collected and handled prior to testing can significantly impact the measurements obtained from that sample. They understand that data from poorly handled samples will likely harm the data set when developing scientific associations and conclusions. For example, not correcting for flow rate when measuring an analyte that is impacted by flow, using an incorrect method to stimulate flow, using samples subjected to multiple freeze-thaws, or using an unvalidated swab for the collection of saliva without fully understanding the bias/interference created by the swab material (See Part 2, How Good are Your Saliva Samples?) are all errors that may affect scientific conclusions. Conversely, following validated methods reduces variability: from collection; from transport; from storage; from freezing; from the bench – all of which increase the repeatability of the results. A high-quality lab knows what the validated saliva collection methods are, and like Salimetrics, they know how to make the best saliva collection recommendation for a study. These labs keep up with evolving best practices and are a great resource for newer researchers.

EASY & ACCURATE

Saliva Panel and Profiles

Salivary Cytokine Panel

Salivary DLMO Profile

Target Analyte(s): Salivary Melatonin
Optimum Collection Volume: 500 μL/sample
Sample Test Volume: 100 μL
Collect With: SalivaBio Passive Drool Method
SalivaBio Oral Swab (SOS) Method
SalivaBio Children's Swab (SCS) Method
SalivaBio Infant's Swab (SIS) Method
Assay Sensitivity: 1.37 pg/mL
Assay Range: 0.78-50 pg/mL
Serum-Saliva Correlation: 0.81

Salivary Analytes

Salivary 17 Alpha-OHP

Optimum Collection Volume: 125 μL*
Sample Test Volume: 50 μL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 3 pg/mL
Assay Range: 5.1 pg/mL - 500pg/mL
Serum-Saliva Correlation: 0.64

Salivary Aldosterone

Optimum Collection Volume: 175 μL*
Sample Test Volume: 100 µl
Collect With: SalivaBio Oral Swab (SOS) Method
SalivaBio Children's Swab (SCS) Method
SalivaBio Infant's Swab (SIS) Method
SalivaBio Passive Drool Method
Assay Sensitivity: 35 pg/mL
Assay Range: -----
Serum-Saliva Correlation: 0.75-0.96

Salivary Alpha-Amylase

Optimum Collection Volume: 25 μL*
Sample Test Volume: 10 µL of saliva then 8 µL of X200 dilution
Collect With: SalivaBio Passive Drool Method
SalivaBio Oral Swab (SOS) Method
SalivaBio Children's Swab (SCS) Method
SalivaBio Infant's Swab (SIS) Method
Assay Sensitivity: 0.4 U/mL
Assay Range: 2-400 U/mL
Serum-Saliva Correlation: NA

Salivary Androstenedione

Optimum Collection Volume: 125 μL*
Sample Test Volume: 50 µL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 5 pg/mL
Assay Range: 10 pg/mL - 2430 pg/mL
Serum-Saliva Correlation: NA

Salivary C-Reactive Protein

Optimum Collection Volume: 225 μL*
Sample Test Volume: 150 µL of saliva, 100 µL of x2 dilution
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 9.72 pg/mL
Assay Range: 25 pg/mL - 1600 pg/mL
Serum-Saliva Correlation: NA

Salivary Chromogranin A

Optimum Collection Volume: 75 μL*
Sample Test Volume: 25 μL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 7 ng/mL
Assay Range: NA
Serum-Saliva Correlation: NA

Salivary Cortisol

Optimum Collection Volume: 75 uL
Sample Test Volume: 25 μL
Collect With: SalivaBio Passive Drool Method
SalivaBio Oral Swab (SOS) Method
SalivaBio Children's Swab (SCS) Method
SalivaBio Infant's Swab (SIS) Method
Assay Sensitivity: <0.007 ug/dL
Assay Range: 0.012-3.000 ug/dL
Serum-Saliva Correlation: 0.91

Salivary Cotinine

Optimum Collection Volume: 75 µL*
Sample Test Volume: 20 µL
Collect With: SalivaBio Passive Drool Method
SalivaBio Oral Swab (SOS) Method
SalivaBio Children's Swab (SCS) Method
SalivaBio Infant's Swab (SIS) Method
Assay Sensitivity: 0.15 ng/mL
Assay Range: 0.8 ng/mL - 200 ng/mL
Serum-Saliva Correlation: NA

Salivary DHEA

Optimum Collection Volume: 125 μL*
Sample Test Volume: 50 µL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 5 pg/mL
Assay Range: 10.2 pg/mL - 1000 pg/mL
Serum-Saliva Correlation: 0.86

Salivary DHEA-S

Optimum Collection Volume: 225 μL*
Sample Test Volume: 100 µL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 43 pg/mL
Assay Range: 188.9 pg/mL- 15,300 pg/mL
Serum-Saliva Correlation: NA

Salivary Estradiol

Optimum Collection Volume: 225 μL*
Sample Test Volume: 100 µL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 0.1 pg/mL
Assay Range: 1 pg/mL - 32 pg/mL
Serum-Saliva Correlation: 0.80

Salivary Estriol

Optimum Collection Volume: 175 μL*
Sample Test Volume: 100 µL of X2 dilution
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 1 pg/mL
Assay Range: 5 pg/mL -4860 pg/mL
Serum-Saliva Correlation: 0.87

Salivary Estrone

Optimum Collection Volume: 255 μL*
Sample Test Volume: 100 µL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 1 pg/mL
Assay Range: 3.1 pg/mL - 300 pg/mL
Serum-Saliva Correlation: NA

Salivary IgG

Optimum Collection Volume: 50 μL*
Sample Test Volume: 10 uL
Collect With: SalivaBio Passive Drool Method
SalivaBio Oral Swab (SOS) Method
SalivaBio Children's Swab (SCS) Method
SalivaBio Infant's Swab (SIS) Method
Assay Sensitivity: 0.94 µg/mL
Assay Range: 0.3125 - 20 ng/mL
Serum-Saliva Correlation: NA

Salivary IgM

Optimum Collection Volume: 50 μL*
Sample Test Volume: 10 uL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 0.03 ng/mL
Assay Range: 0.39 – 25 ng/mL
Serum-Saliva Correlation: NA

Salivary Insulin

Optimum Collection Volume: 100 μL
Sample Test Volume: 25 μL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 7.5 pg/mL
Assay Range: 3.2-50,000 pg/mL
Serum-Saliva Correlation: 0.92

Salivary Interferon Gamma

Optimum Collection Volume: 100 μL
Sample Test Volume: 25 μL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 0.37 pg/mL
Assay Range: 0.37 – 4760 pg/mL
Serum-Saliva Correlation: NA

Salivary Interleukin-1 Beta

Optimum Collection Volume: 50 μL*
Sample Test Volume: 20 μL
Collect With: SalivaBio Passive Drool Method
SalivaBio Oral Swab (SOS) Method
SalivaBio Children's Swab (SCS) Method
SalivaBio Infant's Swab (SIS) Method
Assay Sensitivity: 0.37 pg/mL
Assay Range: 3.13 pg/mL - 200 pg/mL
Serum-Saliva Correlation: NA

Salivary Interleukin-10

Optimum Collection Volume: 100 μL
Sample Test Volume: 25 μL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 0.04 pg/mL
Assay Range: 0.04 – 1420 pg/m
Serum-Saliva Correlation: NA

Salivary Interleukin-12p70

Optimum Collection Volume: 100 μL
Sample Test Volume: 25 μL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 0.11 pg/mL
Assay Range: 0.11 – 2180 pg/mL
Serum-Saliva Correlation:

Salivary Interleukin-13

Optimum Collection Volume: 100 μL
Sample Test Volume: 25 μL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 0.24 pg/mL
Assay Range: 0.24 – 2072 pg/mL
Serum-Saliva Correlation: NA

Salivary ​Interleukin-2

Optimum Collection Volume: 100 μL
Sample Test Volume: 25 μL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 0.09 pg/mL
Assay Range: 0.09 – 6040 pg/mL
Serum-Saliva Correlation:

Salivary Interleukin-6

Optimum Collection Volume: 135 μL
Sample Test Volume: 60 μL
Collect With: SalivaBio Passive Drool Method
SalivaBio Oral Swab (SOS) Method
SalivaBio Children's Swab (SCS) Method
SalivaBio Infant's Swab (SIS) Method
Assay Sensitivity: 0.07 pg/mL
Assay Range: 0 – 100 pg/mL
Serum-Saliva Correlation: NA

Salivary Interleukin-8

Optimum Collection Volume: 100 μL
Sample Test Volume: 25 μL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 0.07 pg/mL
Assay Range: 0.07 – 2336 pg/mL
Serum-Saliva Correlation: NA

Salivary Melatonin

Optimum Collection Volume: 225 μL*
Sample Test Volume: 100 µL
Collect With: SalivaBio Passive Drool Method
SalivaBio Oral Swab (SOS) Method
SalivaBio Children's Swab (SCS) Method
SalivaBio Infant's Swab (SIS) Method
Assay Sensitivity: 1.37 pg/mL
Assay Range: 0.78-50 pg/mL
Serum-Saliva Correlation: 0.81

Salivary Neopterin

Optimum Collection Volume: 125 μL*
Sample Test Volume: 50 μL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 0.51 ng/mL
Assay Range: -----
Serum-Saliva Correlation: NA

Salivary Osteocalcin

Optimum Collection Volume: 150 μL
Sample Test Volume: 25 μL
Collect With: SalivaBio Passive Drool Method
SalivaBio Oral Swab (SOS) Method
SalivaBio Children's Swab (SCS) Method
SalivaBio Infant's Swab (SIS) Method
Assay Sensitivity: 9.77 pg/mL
Assay Range: 9.77 - 10,000 pg/mL
Serum-Saliva Correlation: N/A

Salivary Oxytocin

Optimum Collection Volume: 150 μL
Sample Test Volume: 25 μL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 8 pg/mL
Assay Range: 8-1000 pg/mL
Serum-Saliva Correlation: N/A

Salivary Progesterone

Optimum Collection Volume: 125 μL*
Sample Test Volume: 50 μL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 5 pg/mL
Assay Range: 10 pg/mL - 2430 pg/mL
Serum-Saliva Correlation: NA

Salivary SARS-CoV-2 (N) IgG

Optimum Collection Volume: 200 μL
Sample Test Volume: 50 uL
Collect With: SalivaBio Passive Drool Method
SalivaBio Oral Swab (SOS) Method
SalivaBio Children's Swab (SCS) Method
SalivaBio Infant's Swab (SIS) Method
Assay Sensitivity: 92%
Assay Sensitivity: 97.6%
Assay Range: Qualitative
Serum-Saliva Correlation: NA

Salivary SIgA

Optimum Collection Volume: 50 μL*
Sample Test Volume: 25 μL
Collect With: SalivaBio Passive Drool Method
SalivaBio Oral Swab (SOS) Method
SalivaBio Children's Swab (SCS) Method
SalivaBio Infant's Swab (SIS) Method
Assay Sensitivity: 2.5 μg/mL
Assay Range: 2.50 µg/mL – 600 µg/mL
Serum-Saliva Correlation:

Salivary Testosterone

Optimum Collection Volume: 75 μL*
Sample Test Volume: 25 μL
Collect With: SalivaBio Passive Drool Method
SalivaBio Oral Swab (SOS) Method
SalivaBio Children's Swab (SCS) Method
SalivaBio Infant's Swab (SIS) Method
Assay Sensitivity: 1 pg/mL
Assay Range: 6.1 pg/mL - 600 pg/mL
Serum-Saliva Correlation: 0.96

Salivary TNF-Alpha

Optimum Collection Volume: 425 μL*
Sample Test Volume: 200 μL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 0.106 pg/mL
Assay Range: 0.04 - 1360 pg/mL
Serum-Saliva Correlation: NA

Salivary Total Protein

Optimum Collection Volume: 75 μL*
Sample Test Volume: 25 μL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 5 μg/mL
Assay Range: -----
Serum-Saliva Correlation: NA

Salivary Transferrin

Optimum Collection Volume: 75 μL*
Sample Test Volume: 20 µL
Collect With: SalivaBio Passive Drool Method
Assay Sensitivity: 0.08 mg/dL
Assay Range: 0.08 mg/dL - 6.6 mg/dL
Serum-Saliva Correlation: NA

Salivary Uric Acid

Optimum Collection Volume: 25 μL*
Sample Test Volume: 10 µL
Collect With: SalivaBio Passive Drool Method
SalivaBio Oral Swab (SOS) Method
SalivaBio Children's Swab (SCS) Method
SalivaBio Infant's Swab (SIS) Method
Assay Sensitivity: 0.07 mg/dL
Assay Range: 0.07- 20 mg/dL
Serum-Saliva Correlation: 0.84

Add DNA Analysis to My Study

Considerations for adding Salivary DNA to analyte Studies:

You can combine salivary analytes with easy, accurate, and affordable genomic testing using Salimetrics SalivaLab and the same sample that you are already collecting – no specialized saliva collection devices or additional samples are required.

Don’t know what SNPs are right for you? The SalivaLab’s DNA team specializes in genetic testing services, we recommend you Request a DNA Consult (gratis) to learn more about common considerations such as # of samples, participant ethnicity, and IRB Approval.

All DNA Services

DNA Extraction and Normalization
Single Nucleotide Polymorphism (SNP) Genotyping
VNTR & STR Analysis


Clinical and Diagnostics


Industry-Leading Clinical Research Services for Saliva Analysis Integration

The Salimetrics SalivaLab is a CLIA certified lab which has been supporting the drug discovery & development process of pharmaceutical and contract research organizations for over ten years. Salimetrics has standardized the tools necessary to provide cost-effective, biological measurements for saliva biomarkers and analytes in your biomedical, pharmacological, or clinical research project. The SalivaLab provides unparalleled saliva analysis in support of your therapeutic area of expertise.

Our Approach
The Salimetrics platform gives you flexibility for incorporating salivary biomarkers into your drug development or clinical research program. From early stage drug discovery, target validation, non-clinical/pre-clinical pharmacokinetic/pharmacodynamic studies, and through all phases of clinical trials, Salimetrics can provide your team with valuable biological data with saliva-based bioanalytics. Our saliva research team can assist clinical investigators in designing, participant screening or therapeutic drug monitoring to achieve your investigational research goals.

Getting Started

If you’re interested in incorporating salivary analytes or biomarkers into your drug discovery program, the fastest way to reach us is to schedule a consultation by calling us at:800.790.2258

Both large and small drug discovery projects can benefit from enhanced salivary biomarker services including:

  • Priority Testing
  • Flexibility to meet changing needs
  • Validation Assistance
  • Specialized collection and assay training
  • Responsive Salivary Specialist for project inquiries
  • Logistical management
  • Secure network specific File Transfer Protocol (FTP) for data
  • Specimen shipping/ tracking
  • Custom collection supplies
  • Assistance with project design
Contact: Salimetrics (USA)
View All International Distributors

How to Collect Saliva for Covid-19 Testing

Collecting a saliva sample for COVID-19 testing is simple and easy to do with our Saliva Collection Methods. Relax – that’s the first step! Then watch one of these video collection instructions prior to starting your collection:

Saliva Collection Aid – Standard, 48-Format
Saliva Collection Aid – Narrow, 96-Well Format
Oral Swab with Syringe for Onsite Testing
Oral Swab with Storage Tube for Lab Testing

CALL 800.790.2258
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