Levels of SIgA in saliva vary in response to physical and psychological stress through interactions with the autonomic nervous system. (5,6) SIgA levels in saliva are affected by flow rates, with concentrations normally decreasing as flow rates increase. Measurement of flow rates is advisable in order to express SIgA secretion as a function of time. (6)
Technical Summary
| Analyte Summary | |
|---|---|
| Analyte: | Secretory Immunoglobulin A |
| Aliases: | SIgA, Secretory IgA |
| Serum-Saliva Correlation: | NA |
| Optimum Collection Volume: | 50 μL* |
| Special Considerations |
|---|
| SIgA is Location Dependent SIgA is Flow Rate Dependent (μg/mL) Passive Drool is the Recommended Collection Method |
| Assay Summary | |
|---|---|
| Methodology: | ELISA |
| Sensitivity: | 2.5 μg/mL |
| Assay Range: | 2.50 µg/mL – 600 µg/mL |
| Assay Type: | Quantitative |
Collect Saliva Samples
SECRETORY IgA SALIVA COLLECTION CONSIDERATIONS
Better results begin with better saliva collection. This collection protocol features general considerations to maximize salivary Secretory IgA analysis. Use this analyte specific collection protocol to plan your collection methodology and sampling schemes.
APPROVED SALIVARY SECRETORY IgA COLLECTION METHODS
Recommended
Test Saliva Samples
| Order Code (lab): | 5180 |
| Transport Requirements: | Ship on Dry Ice |
Add DNA Analysis to My Study
Considerations for adding Salivary DNA to analyte Studies:
You can combine salivary analytes with easy, accurate, and affordable genomic testing using Salimetrics SalivaLab and the same sample that you are already collecting – no specialized saliva collection devices or additional samples are required.
Don’t know what SNPs are right for you? The SalivaLab’s DNA team specializes in genetic testing services, we recommend you Request a DNA Consult (gratis) to learn more about common considerations such as # of samples, participant ethnicity, and IRB Approval.
All DNA Services
DNA Extraction and Normalization
Single Nucleotide Polymorphism (SNP) Genotyping
VNTR & STR Analysis
References & Salivary Secretory IgA Research
- Holmgren, J., Czerkinsky, C. (2005). Mucosal immunity and vaccines. Nature Medicine, 11(4 Suppl), s45-53.
- Brandtzaeg, P. (2007). Do salivary antibodies reliably reflect both mucosal and systemic immunity? Ann N Y Acad Sci, 1098, 288-311.
- Crawford, J.M., Taubman, M.A., Smith D.J. (1975). Minor salivary glands as a major source of secretory immunoglobulin A in the human oral cavity. Science, 190(4220), 1206-9.
- Li, T.-L., Gleeson, M. (2004). The effect of single and repeated bouts of prolonged cycling and circadian variation on saliva flow rate, immunoglobulin A and alpha-amylase responses. J Sports Sci, 22(11-12), 1015-1024.
- Tsujita, S., Morimoto, K. (1999). Secretory IgA in saliva can be a useful stress marker. Env Health Prev Med, 4, 1-8.
- Bishop, N.C., Gleeson, M. (2009). Acute and chronic effects of exercise on markers of mucosal immunity. Front Biosci, 1(14), 4444-56.