In 2008, Thorgeirsson et al. highlighted a connection between CHRNA3 and nicotine addiction by drawing a strong association between the T allele variant of SNP rs1051730 and the quantity of cigarettes smoked per day. Their findings also support that those with the variant have higher rates of nicotine dependence (2).
Multiple studies have since confirmed the connection between SNP rs1051730 and COPD, lung cancer, and nicotine dependence (3,4,5,6). A meta-analyses performed by Cui et al. positively linked 4 SNPs from the CHRNA3/5 locus to chronic obstructive pulmonary disease (COPD) and of the evaluated SNPs (rs1051730, rs8034191, rs6495309, and rs16969968) rs1051730 showed the most significant association to COPD in non-Asians (4). Zhang et al. 2011, also revealed a susceptibility connection between rs1051730 and COPD as it relates to airway obstruction and parenchyma destruction (5).
In 2012 Yang et al. looked at patients from southern and eastern Chinese populations and concluded that SNP rs6495309 showed a significant association with both COPD and lung cancer. Individuals with this polymorphism were homozygous for the CC alleles and therefore at an increased risk for COPD, while individuals with the heterozygous alleles (CT/CC) showed a risk for lung cancer (6). For more details on CHRNA3 visit SNPedia here.
When interpreting data from your current study or planning for the future, consider that there are many polymorphisms that influence brain function and nicotine addiction. For a complete list of SNPs available for research, we recommend contacting one of our experts for guidance on the most relevant selections for your research.
|Gene:||Cholinergic Receptor, Nicotinic Alpha 3|
|Related Analyte(s):||Cotinine, Cortisol|
|Genomic DNA, purified using silica based membrane columns is analyzed by TaqMan® SNP Genotyping Assays run on the 7900HT real time PCR system
Yield: 2-5 ug genomic DNA
Collect Saliva Samples
DNA SALIVA COLLECTION CONSIDERATIONS
Better results begin with better saliva collection. This collection protocol features general considerations to maximize salivary DNA analysis. Use this collection protocol to plan your collection methodology and sampling schemes.
APPROVED SALIVARY DNA COLLECTION METHODS
Test Saliva Samples
|Order Code (lab):||5811 (SNP Genotyping)|
|Transport Requirements:||Ship on Dry Ice|
The validated method used by Salimetrics is proprietary and not available in assay kit form at this time.
References & CHRNA3 Related Research
- CHRNA3 cholinergic receptor nicotinic alpha 3 on NCBI
- Thorgeirsson, TE. et al. (2008). A variant associated with nicotine dependence, lung cancer and peripheral arterial disease. Nature. 452(7187), 638-642.
- Pillai, S. et al. (2009). A Genome-Wide Association Study in Chronic Obstructive Pulmonary Disease (COPD): Identification of Two Major Susceptibility Loci. PLOS Genetics. 5(3), e1000421.
- Cui, K. et al. (2014). Four SNPs in the CHRNA3/5 alpha-neuronal nicotinic acetylcholine receptor subunit locus are associated with COPD risk based on meta-analyses. PLOS ONE. 9(7), e102324.
- Zhang, J. et al. (2011). Nicotinic acetylcholine receptor variants associated with susceptibility to chronic obstructive pulmonary disease: a meta-analysis. Respiratory Research. 12(1), 158.
- Yang, L. et al. (2012). Functional Polymorphisms of CHRNA3 Predict Risks of Chronic Obstructive Pulmonary Disease and Lung Cancer in Chinese. PLOS ONE. 7(10):e46071.