Visual inspection of saliva samples is not adequate to detect blood contamination, since microinjuries to blood vessel membranes may allow hormone molecules and proteins to pass through while still retaining the much larger red blood cells. Dipstick tests for blood, which look for the presence of hemoglobin, are also not a reliable means of screening saliva samples, due to the presence of peroxidases in saliva, which can generate false positive results. (2,3)
The Salimetrics Salivary Blood Contamination Enzyme Immunoassay quantitatively measures transferrin, a large protein (mol wt 76,000) present in abundance in blood that is normally present in only trace amounts in saliva. Higher levels of transferrin measured in saliva indicate the presence of blood contamination and serve as a warning to investigators that samples should be excluded from subsequent quantitative assays for salivary analytes and statistical analyses. (2,3,4) Saliva samples collected from populations that have little or no dental care, or known oral health problems, are especially appropriate for screening with the Blood Contamination Assay Kit.
|Optimum Test Volume:||75 μL*|
|Assay Range:||0.08 mg/dL - 6.6 mg/dL|
Collect Saliva Samples
TRANSFERRIN SALIVA COLLECTION CONSIDERATIONS
High levels of transferrin measured in saliva indicate the presence of blood contamination and serve as a warning to investigators that samples should be excluded from subsequent quantitative assays for salivary analytes and statistical analyses. Saliva samples collected from populations that have little or no dental care, or known oral health problems, are especially appropriate for screening with the Blood Contamination Assay Kit. To screen your samples for blood contamination, we recommend an optimum collection volume of 75 μL in addition to the volume required to perform analysis on your analytes of interest.
Test Saliva Samples
|Order Code (lab):||5300|
|Transport Requirements:||Ship on Dry Ice|
The validated method used by Salimetrics is proprietary and not available in assay kit form at this time.
Add DNA Analysis to My Study
Considerations for adding Salivary DNA to analyte Studies:
You can combine salivary analytes with easy, accurate, and affordable genomic testing using Salimetrics SalivaLab and the same sample that you are already collecting – no specialized saliva collection devices or additional samples are required.
Don’t know what SNPs are right for you? The SalivaLab’s DNA team specializes in genetic testing services, we recommend you Request a DNA Consult (gratis) to learn more about common considerations such as # of samples, participant ethnicity, and IRB Approval.
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References & Salivary Transferrin Research
- Malamud, D., & Tabak, L. (Eds.). (1993). Saliva as a diagnostic fluid. Ann N Y Acad Sci, (Vol. 694). New York, NY.
- Kivlighan, K.T., Granger, D.A., Schwartz, E.B., et al. (2004). Quantifying blood leakage into the oral mucosa and its effects on the measurement of cortisol, dehydroepiandrosterone, and testosterone in saliva. Horm Behav, 46(1), 39-46.
- Schwartz, E., & Granger, D.A. (2004). Transferrin enzyme immunoassay for quantitative monitoring of blood contamination in saliva. Clin Chem, 50(3), 654-656.
- Granger, D.A., Cicchetti, D., Rogosch, F., et al. (2007). Blood contamination in children’s saliva: Prevalence, stability, and impact on the measurement of salivary cortisol, testosterone, and dehydroepiandrosterone. Psychoneuroendocrinology, 32(6), 724-33.