In premenopausal women estrone is primarily produced by conversion of androstenedione in the ovaries, with concentrations peaking in the preovulatory phase and a smaller secondary increase during the luteal phase. (1,2) In post-menopausal women, children, and men, estrone is largely produced by conversion of androstenedione in peripheral tissues. (1,2,3) Estrone is subsequently reduced to estradiol in various peripheral tissues. (3)
In non-pregnant women, only about 3% of estrone in the bloodstream is not bound to proteins. (4) Unbound estrone enters saliva from blood via intracellular mechanisms, and in saliva the majority of the estrone remains unbound to protein. The saliva and plasma values are highly correlated. (5)
|Optimum Collection Volume:||255 μL*|
|Assay Range:||3.1 pg/mL - 300 pg/mL|
Collect Saliva Samples
ESTRONE SALIVA COLLECTION CONSIDERATIONS
Better results begin with better saliva collection. This collection protocol features general considerations to maximize salivary Estrone analysis. Use this analyte specific collection protocol to plan your collection methodology and sampling schemes.
Add DNA Analysis to My Study
Considerations for adding Salivary DNA to analyte Studies:
You can combine salivary analytes with easy, accurate, and affordable genomic testing using Salimetrics SalivaLab and the same sample that you are already collecting – no specialized saliva collection devices or additional samples are required.
Don’t know what SNPs are right for you? The SalivaLab’s DNA team specializes in genetic testing services, we recommend you Request a DNA Consult (gratis) to learn more about common considerations such as # of samples, participant ethnicity, and IRB Approval.
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References & Salivary Estrone Research
- Faiman, C., Winter, J.S., Reyes, F.I. (1976). Patterns of gonadotrophins and gonadal steroids throughout life. Clin Obstet Gynecol, 3(3), 467-83.
- Cañez, M.S., Lee, K.J., & Olive, D.L. (1992). Progestogens and estrogens. Infert Reprod Med Clin North Amer, 3, 59-78.
- Sasano, H., Harada, N. (1998). Intratumoral aromatase in human breast, endometrial, and ovarian malignancies. Endocr Rev, 19(5), 593-607.
- Speight, A.C., Hancock, K.W., Oakey, R.E. (1979). Non-protein-bound oestrogens in plasma and urinary excretion of unconjugated oestrogens in non-pregnant women. J Endocrinol, 83, 385-91.
- Vining, R.F., McGinley, R.A. (1987). The measurement of hormones in saliva: Possibilities and pitfalls. J Steroid Biochem, 27(1-3), 81-94.