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Salivary Estrone

Salivary Estrone Quick Start Research Guide

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Biomarkers

1. How to collect Salivary Estrone

APPROVED SALIVARY ESTRONE COLLECTION METHODS

Passive Drool Saliva Collection Kit

SalivaBio Passive Drool Method

Use With: Adults, Children 6+

Salivary Estrone Collection Protocol

Collection volume, general considerations, and basic guidelines to maximize salivary estrone sample integrity. Use this analyte-specific collection protocol to plan you collection methodology and sampling schemes.

Biomarkers

2. How to Assay for Salivary Estrone

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Order Code5206

Assay Protocol
Rev. 04.19.19
Specifications
Catalog#: 1-3202
Regulatory Status: RUO
Format: 96-well plate
Assay Time: ~ 4 hrs
Sample Volume/Test: 100 µL
Sensitivity: 1 pg/mL
Assay Range: 3.1 pg/mL - 300 pg/mL
Storage Requirements: 2-8°C
Tests Per Kit
Singlet: 76
Duplicate: 38
Target Analyte

Technical Documentation

Assay Kit Overview

Intended Use

The Salimetrics Estrone Enzyme Immunoassay Kit is a competitive immunoassay specifically designed and validated for the quantitative measurement of salivary Estrone. It is not intended for diagnostic use. It is intended only for research use in humans and some animals. Salimetrics has not validated this kit for serum or plasma samples.

Introduction

Estrone [3-hydroxy-1,3,5(10)-estratrien-17-one; E1] is a naturally occurring steroidal hormone. A major portion of Estrone is produced from androstenedione in prepubertal children, men, and postmenopausal women. Circulating Estrone levels are relatively high at birth in both males and females, decrease postnatally, and increase during puberty. Of the three major estrogens, Estrone is predominant after menopause in women. Estrone is primarily secreted by the ovaries in premenopausal women, peaking in the preovulatory phase with a smaller secondary increase during the luteal phase. Estrone is a primary component of many pharmaceutical preparations. Research concerning Estrone is often focused on pregnancy, reproduction, and menopause. However, estrogens affect a diverse group of biological processes such as arterial vasodilation, bone density, cognitive function, and neuroprotection. Estrogens are also studied in regard to coronary artery disease, immunocompetence, cancer susceptibility and polycystic ovarian syndrome.

Salivary Estrone Assay Principle

This is a competitive immunoassay kit. Estrone in standards and samples compete with Estrone conjugated to horseradish peroxidase for the antibody binding sites on a microtitre plate. After incubation, unbound components are washed away. Bound Estrone Enzyme Conjugate is measured by the reaction of the horseradish peroxidase enzyme to the substrate tetramethylbenzidine (TMB). This reaction produces a blue color. A yellow color is formed after stopping the reaction with an acidic solution. The optical density is read on a standard plate reader at 450 nm. The amount of Estrone Enzyme Conjugate detected is inversely proportional to the amount of Estrone present in the sample.

Biomarkers

3. Technical Summary

Analyte Summary
Analyte: ​Estrone
Aliases: 3-hydroxy-1,3,5(10)-estratrien-17-one, E1
Serum-Saliva Correlation: NA
Optimum Collection Volume: 255 μL*
*Add 300 µl to the total volume of all tests for liquid handling
Assay Summary
Methodology: ELISA
Sensitivity: 1 pg/mL
Assay Range: 3.1 pg/mL - 300 pg/mL
Assay Type: Quantitative

Background

​Estrone (3-hydroxy-1,3,5(10)-estratrien-17-one; E1) is one of the three main estrogenic steroid hormones produced in humans. Circulating estrone levels are relatively high at birth in both males and females, decrease postnatally, and increase during puberty. (1) Of the three major estrogens, estrone is predominant after menopause in women. (1) In premenopausal women estrone is primarily produced by conversion of androstenedione in the ovaries, with concentrations peaking in the preovulatory phase and a smaller secondary increase during the luteal phase. (1,2) In post-menopausal women, children, and men, estrone is largely produced by conversion of androstenedione in peripheral tissues. (1,2,3) Estrone is subsequently reduced to estradiol in various peripheral tissues. (3) In non-pregnant women, only about 3% of estrone in the bloodstream is not bound to proteins. (4) Unbound estrone enters saliva from blood via intracellular mechanisms, and in saliva the majority of the estrone remains unbound to protein. The saliva and plasma values are highly correlated. (5) ​

References & Salivary Estrone Research

  1. Faiman, C., Winter, J.S., Reyes, F.I. (1976). Patterns of gonadotrophins and gonadal steroids throughout life. Clin Obstet Gynecol, 3(3), 467-83.
  2. Cañez, M.S., Lee, K.J., & Olive, D.L. (1992). Progestogens and estrogens. Infert  Reprod Med Clin North Amer, 3, 59-78.
  3. Sasano, H., Harada, N. (1998). Intratumoral aromatase in human breast, endometrial, and ovarian malignancies.  Endocr Rev, 19(5), 593-607.
  4. Speight, A.C., Hancock, K.W., Oakey, R.E. (1979).  Non-protein-bound oestrogens in plasma and urinary excretion of unconjugated oestrogens in non-pregnant women.  J Endocrinol, 83, 385-91.
  5. Vining, R.F., McGinley, R.A. (1987). The measurement of hormones in saliva: Possibilities and pitfalls.  J Steroid Biochem, 27(1-3), 81-94.

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