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Salivary Testosterone

Technical Summary

Analyte Summary:
Analyte: ​Testosterone
Aliases: Androst-4-en-17β-ol-3-one
Serum-Saliva Correlation: 0.96
Optimum Collection Volume: 75 μL*
*Add 300 µl to the total volume of all tests for liquid handling
Assay Summary:
Methodology: ELISA
Sensitivity: 1 pg/mL
Assay Range: 6.1 pg/mL - 600 pg/mL
Assay Type: Quantitative

Collect Saliva Samples


Better results begin with better saliva collection. This collection protocol features general considerations to maximize salivary ​Testosterone analysis. Use this analyte specific collection protocol to plan your collection methodology and sampling schemes.

Test Saliva Samples

@ Salimetrics
Salimetrics SalivaLab - Easy & Accurate
Order Code (lab): 5138
Transport Requirements: Ship on Dry Ice

Add DNA Analysis to My Study

Considerations for adding Salivary DNA to analyte Studies:

You can combine salivary analytes with easy, accurate, and affordable genomic testing using Salimetrics SalivaLab and the same sample that you are already collecting – no specialized saliva collection devices or additional samples are required.

Don’t know what SNPs are right for you? The SalivaLab’s DNA team specializes in genetic testing services, we recommend you Request a DNA Consult (gratis) to learn more about common considerations such as # of samples, participant ethnicity, and IRB Approval.

All DNA Services

DNA Extraction and Normalization
Single Nucleotide Polymorphism (SNP) Genotyping
VNTR & STR Analysis

References & Salivary ​Testosterone Research

    1. Labrie, F., Luu-The, V., Bélanger, A., et al. (2005).  Is dehydroepiandrosterone a hormone? J Endocrinol, 187(2), 169-96.
    2. Nakamura, Y., Hornsby, P.J., Casson, P., et al. (2008).  Type 5 17β-hydroxysteroid dehydrogenase (AKR1C3) contributes to testosterone production in the adrenal reticularis. J Clin Endocrinol Metab, 94(6), 2192-98.
    3. Burger, H.G. (2002). Androgen production in women. Fertil Steril, 77(Suppl 4), S3-5.
    4. Labrie, F., Bélanger, A., Cusan, L., Candas, B. (1997).  Physiological changes in dehydroepiandrosterone are not reflected by serum levels of active androgens and estrogens but of their metabolites: Intracrinology.  J Clin Endocrinol Metab, 82(8), 2403-9.
    5. Ankarberg, C., Norjavaara, E. (1999).  Diurnal rhythm of testosterone secretion before and throughout puberty in healthy girls: Correlation with 17β-estradiol and dehydroepiandrosterone sulfate.  J Clin Endocrinol Metab, 84(3), 975-84.
    6. Diver, M.J., Imtiaz, K.E., Ahmad, A.M., et al. (2003).  Diurnal rhythms of serum total, free and bioavailable testosterone and of SHBG in middle-aged men compared with those in young men. Clin Endocrinol (Oxf), 58(6), 710-17.
    7. Rogol, A.D., Clark, P.A., Roemmich, J.N. (2000).  Growth and pubertal development in children and adolescents: Effects of diet and physical activity.  Am J Clin Nutr, 72(2 Suppl.), 521S-28.
    8. Snyder, P.J., Peachey, H., Berlin, J.A., et al. (2000). Effects of testosterone replacement in hypogonadal men.  J Clin Endocinol Metab, 85(8), 2670-77.
    9. Tibblin, G., Adlerberth, A., Lindstedt, G., Björntorp, P. (1996).  The pituitary-gonadal axis and health in elderly men: A study of men born in 1913.  Diabetes, 45(11), 1605-9.
    10. Davis, S.R., Tran, J. (2001). Testosterone influences libido and well being in women. Trends Endocrinol Metab, 12(1), 33-7.

    1. Wang, C., Alexander, G., Berman, N., et al. (1996). Testosterone replacement therapy improves mood in hypogonadal men: A clinical research center study. J Clin Endocrinol Metab, 81(10), 3578-83.
    2. Malkin, C.J., Pugh, P.J., West, J.N., et al. (2006). Testosterone therapy in men with moderate severity heart failure: A double-blind randomized placebo controlled trial.  Eur Heart J, 27(1), 57-64.
    3. Bhasin, S., Bremner, W.J. (1997). Clinical review 85: Emerging issues in androgen replacement therapy.  J Clin Endocrinol Metab, 82(1), 3-8.
    4. Gibson, M., Lackritz, R., Schiff, I., Tulchinsky, D. (1980). Abnormal adrenal responses to adrenocorticotropic hormone in hyperandrogenic women.  Fertil Steril, 33(1), 43-8.
    5. Rodin, A., Thakkar, H., Taylor, N., Clayton, R. (1994). Hyperandrogenism in polycystic ovary syndrome: Evidence of dysregulation of 11β-hydroxysteroid dehydrogenase.  N Eng J Med, 330(7), 460-65.
    6. Vining, R.F., MicGinley, R.A. (1987). The measurement of hormones in saliva: Possibilities and pitfalls.  J Steroid Biochem, 27(1-3), 81-94.
    7. Wang, C., Plymate, S., Nieschlag, E., Paulsen, C.A. (1981). Salivary testosterone in men: Further evidence of a direct correlation with free serum testosterone.  J Clin Endocrinol Metab, 53(5), 1021-24.
    8. Rollin, G. (2010). The trials of testosterone testing.  Clin Lab News, 36(8), 1-5.

Contact: Salimetrics (USA)
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