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Salivary Melatonin

Technical Summary

Analyte Summary
Analyte: Melatonin
Aliases: N-acetyl-5-methoxytryptamine
Serum-Saliva Correlation: 0.81
Optimum Collection Volume: 225 μL*
*Add 300 µl to the total volume of all tests for liquid handling
Interfering Factors
Avoid foods such as pitted fruit, bananas and chocolate 24 hours before sample collection.
Assay Summary
Methodology: ELISA
Sensitivity: 1.37 pg/mL
Assay Range: 0.78-50 pg/mL
Assay Type: Quantitative

Collect Saliva Samples


Better results begin with better saliva collection. This collection protocol features general considerations to maximize salivary Melatonin analysis. Use this analyte specific collection protocol to plan your collection methodology and sampling schemes.


Test Saliva Samples

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Salimetrics SalivaLab - Easy & Accurate
Order Code (lab): 5110
Transport Requirements: Ship on Dry Ice
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Add DNA Analysis to My Study

Considerations for adding Salivary DNA to analyte Studies:

You can combine salivary analytes with easy, accurate, and affordable genomic testing using Salimetrics SalivaLab and the same sample that you are already collecting – no specialized saliva collection devices or additional samples are required.

Don’t know what SNPs are right for you? The SalivaLab’s DNA team specializes in genetic testing services, we recommend you Request a DNA Consult (gratis) to learn more about common considerations such as # of samples, participant ethnicity, and IRB Approval.

All DNA Services

DNA Extraction and Normalization
Single Nucleotide Polymorphism (SNP) Genotyping
VNTR & STR Analysis

References & Salivary Melatonin Research

    1. Claustrat, B., Brun, J., Chazot, G. (2005).  The basic physiology and pathophysiology of melatonin.  Sleep Med Rev, 9(1), 11-24.
    2. Cajochen, C., Kräuchi, K., Wirz-Justice, A. (2003).  Role of melatonin in the regulation of human circadian rhythms and sleep.  J Neuroendocrinol, 15(4), 432-37.
    3. Jou, M.-J., Peng, T.-I., Yu, P.-Z., et al. (2007).  Melatonin protects against common deletion of mitochondrial DNA-augmented mitochondrial oxidative stress and apoptosis.  J Pineal Res, 43(4), 389-403.
    4. Rodriguez, C., Mayo, J.C., Sainz, R.M., et al. (2003).  Regulation of antioxidant enzymes: A significant role for melatonin.  J Pineal Res, 36(1), 1-9.
    5. Gupta, Y.K., Gupta, M., Kohli, K. (2003).  Neuroprotective role of melatonin in oxidative stress vulnerable brain.  Indian J Physiol Pharmacol, 47(4), 373-86.
    6. Kennaway, D.J., Voultsios, A. (1998).  Circadian rhythm of free melatonin in human plasma.  J Clin Endocrinol Metab, 83(3), 1013-15.
    7. Gröschl, M., Köhler, H., Topf, H.G., et al. (2008).  Evaluation of saliva collection devices for the analysis of steroids, peptides and therapeutic drugs.  J Pharm Biomed Anal, 47(3), 478-86.
    8. Voultsios, A., Kennaway, D.J., Dawson, D. (1997).  Salivary melatonin as a circadian phase marker: Validation and comparison to plasma melatonin.  J Biol Rhythms, 12(5), 457-66.
    9. Vakkuri, O. (1985).  Diurnal rhythm of melatonin in human saliva.  Acta Physiol Scand, 124(3), 409-412.
    10. Deacon, S., Arendt, J. (1994).  Posture influences melatonin concentrations in plasma and saliva in humans.  Neurosci Lett, 167(1-2), 191-94.

    1. Cutando, A., Gómez-Moreno, G., Villalba, J., et al. (2003).  Relationship between salivary melatonin levels and periodontal status in diabetic patients.  J Pineal Res, 35(4), 239-44.

Contact: Salimetrics (USA)
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