Rs6265, also called Val66Met or G196A, is one of the most commonly studied SNPs along the BDNF genomic loci, and is prevalent in over 19% of Europeans and up to 44% of Asians (4). Increasing research on this SNP is exploring associations between rs6265 and obesity (weight regulation), smoking, depression, introversion, schizophrenia, bipolar disorder, and specific neuropsychiatric diseases. A recent study by Armbruster et al showed that individuals with the met/met homozygotes have a more pronounced cortisol response after a stress test but have a smaller startle response when compared to Val/Val homozygotes or Val/Met heterozygotes (5).
SNP, rs12291063, within the BDNF gene is correlated with decreased BDNF expression and has been associated with increased obesity in both adult and pediatric populations (6). Other commonly studied polymorphisms within the BDNF gene, include C270T, rs7103411, rs2030324, rs2203877, rs2049045 and rs7124442, which are impacting research in the fields of depression, bipolar disorder, PTSD, and schizophrenia. For more information, visit BDNF on SNPedia.
When interpreting data from your current study or planning for the future, consider the polymorphisms that influence brain function. For a complete list of SNPs available for research, we recommend contacting one of our experts for guidance on the most relevant selections for your research.
| Gene Summary | |
|---|---|
| Gene: | Brain-Derived Neurotrophic Factor |
| Gene Symbol: | BDNF |
| Related Analyte(s): | Cortisol |
| Common SNPs |
|---|
| rs6265 , rs12291063 , C270T , rs7103411 , rs2030324 |
| Assay Methodology |
|---|
| Genomic DNA, purified using silica based membrane columns is analyzed by TaqMan® SNP Genotyping Assays run on the 7900HT real time PCR system Yield: 2-5 ug genomic DNA |
| Technical Documentation |
|---|
Collect Saliva Samples
DNA SALIVA COLLECTION CONSIDERATIONS
Better results begin with better saliva collection. This collection protocol features general considerations to maximize salivary DNA analysis. Use this collection protocol to plan your collection methodology and sampling schemes.
APPROVED SALIVARY DNA COLLECTION METHODS
Test Saliva Samples
| Order Code (lab): | 5811 (SNP Genotyping) |
| Transport Requirements: | Ship on Dry Ice |
The validated method used by Salimetrics is proprietary and not available in assay kit form at this time.
References & BDNF Related Research
- Molendijk, ML. et al. (2013). Serum BDNF concentrations as peripheral manifestations of depression: evidence from a systematic review and meta-analyses on 179 associations (N=9484). Molecular Psychiatry. 19(7), 791-800.
- Fernandes, BS. et al. (2013). Decreased peripheral brain-derived neurotrophic factor levels are a biomarker of disease activity in major psychiatric disorders: a comparative meta-analysis. Molecular Psychiatry. 19(7), 750-751.
- Green, MJ. et al. (2011). Brain-derived neurotrophic factor levels in schizophrenia: a systematic review with meta-analysis. Molecular Psychiatry. 16(9), 960-972.
- Petryshen, TL. et al. (2010). Population genetic study of the brain-derived neurotrophic factor (BDNF) gene. Molecular Psychiatry. 15(8), 810-815.
- Armbruster, D. et al. (2015). BDNF val66met genotype shows distinct associations with the acoustic startle reflex and the cortisol stress response in young adults and children. Psychoneuroendocrinology. 66(2015), 39-46.
- Mou, Z. et al. (2015). Human Obesity Associated with an Intronic SNP in the Brain-Derived Neurotrophic Factor Locus. Cell Reports. 13(6), 1073-1080.