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Salivary Cortisol

Technical Summary

Analyte Summary
Analyte: Cortisol
Aliases: hydrocortisone, Compound F
Serum-Saliva Correlation: 0.91
Optimum Collection Volume: 75 μL*
*Add 300 µl to the total volume of all tests for liquid handling
Interfering Factors
Bovine hormones normally present in dairy products can cross-react with anti-cortisol antibodies and cause false results.
Hormone based oral contraceptives and estrogens can cause temporary increase in CBG, potentially lowering cortisol levels
in saliva.
Multiple medications mimic the structure of cortisol and have the potential to influence cortisol levels in saliva.
Assay Summary
Methodology: ELISA
Sensitivity: <0.007 ug/dL
Assay Range: 0.012-3.000 ug/dL
Assay Type: Quantitative
*Salivary Cortisol Example Ranges
Group Number Overall Range (ug/dL)
Children, neonatal 275 ND - 3.417
Children, age 6 months 165 ND - 2.734
Group Number Overall Range (ug/dL)
Normal subjects 19 0.007 - 0.115
Cushing's subjects 21 0.130-2.972
*Note: Examples are to be used as a guide only. Each laboratory should estabilsh its own range.

Collect Saliva Samples

SALIVARY CORTISOL SALIVA COLLECTION CONSIDERATIONS

Better results begin with better analyte collection. This collection protocol features general considerations to maximize salivary cortisol analysis. Use this Analyte-Specific Collection Protocol to plan your collection methodology and sampling schemes.

APPROVED SALIVARY CORTISOL COLLECTION METHODS

Test Saliva Samples

@ Salimetrics
Salimetrics SalivaLab - Easy & Accurate
Order Code (lab): 5100
Transport Requirements: Ship on Dry Ice

Add DNA Analysis to My Study

Considerations for adding Salivary DNA to analyte Studies:

You can combine salivary analytes with easy, accurate, and affordable genomic testing using Salimetrics SalivaLab and the same sample that you are already collecting – no specialized saliva collection devices or additional samples are required.

Don’t know what SNPs are right for you? The SalivaLab’s DNA team specializes in genetic testing services, we recommend you Request a DNA Consult (gratis) to learn more about common considerations such as # of samples, participant ethnicity, and IRB Approval.

All DNA Services

DNA Extraction and Normalization
Single Nucleotide Polymorphism (SNP) Genotyping
VNTR & STR Analysis

References & Salivary Cortisol Research

  1. Migeon, C.J., & Lanes, R.L. (1990). Adrenal cortex: hypo- and hyperfunction. In F. Lifshitz (ed.), Pediatric endocrinology, a clinical guide (2nd ed.), (pp. 333-52). New York: Marcel Dekker.
  2. Drucker, S., New, M.I. (1987).  Disorders of adrenal steroidogenesis. Pediatr Clin North Am, 34(4), 1055-66.
  3. Fischbach, F.T. (1992). The manual of laboratory and diagnostic tests, (4th ed.). Philadelphia: J. B. Lippincott.
  4. Dorn, L.D., Lucke, J.F., Loucks, T.L., Berga, S.L. (2007). Salivary cortisol reflects serum cortisol: analysis of circadian profiles. Ann Clin Biochem, 44(pt 3), 281-84.
  5. Chernow, B., Alexander, H.R., Smallridge, R.C., et al. (1987). Hormonal responses to graded surgical stress. Arch Intern Med, 147(7), 1273-78.
  6. Kreiger, D.T. (1975). Rhythms of ACTH and corticosteroid secretion in health and disease and their experimental modification. J Steroid Biochem, 6(5), 758-91.
  7. Rothfield, B. (1974). Plasma cortisol. In: B. Rothfield (ed.), Nuclear medicine–in vitro (pp. 120-5). Philadelphia: Lippincott.
  8. Robin, P., Predine, J., Milgrom, E. (1977). Assay of unbound cortisol in plasma. J Clin Endocrinol Metab, 46(2), 277-83.
  9. Vining, R.F., McGinley, R.A., Symons, R.G. (1983). Hormones in saliva: mode of entry and consequent implications for clinical interpretation. Clin Chem, 29(10), 1752-56.
  10. Vining, R.F., McGinley, R.A. (1987). The measurement of hormones in saliva: Possibilities and pitfalls. J Steroid Biochem, 27(1-3), 81-94.
  11. Francis, S.J., Walker, R.F., Riad-Fahmy, D., et al. (1987). Assessment of adrenocortical activity in term newborn infants using salivary cortisol determinations. J of Pediatrics, 111, 129-33.
  12. Hiramatsu, R. (1981). Direct assay of cortisol in human saliva by solid phase radioimmunoassay and its clinical applications. Clinica Chimica Acta, 117, 239-249.
  13. Vining, R.F., McGinley, R.A., Maksvytis, J.J., Ho, K.Y. (1983). Salivary cortisol: A better measure of adrenal cortical function than serum cortisol. Ann Clin Biochem, 20(pt 6), 329-35.

How to get started

Contact: Salimetrics (USA)
View All International Distributors

Salimetrics’ COVID-19 Status – 8 April 2020 – No change to Prior Update:

Salimetrics’ Sales and Customer Service, as well as all other non-laboratory personnel, have successfully transitioned to working remote and are fully able to provide support to our customers.

Our Salimetrics Pennsylvania Manufacturing Center remains open for customer orders and shipments. All Salimetrics’ laboratory personnel are following PPE guidelines per CDC Biosafety Level 2 (BSL-2).

The Salimetrics’ SalivaLab, located in California, is open to receive samples. In accordance with State of California Executive Order N-33-20 effective March 19, 2020, our Carlsbad, California facility, including R&D and SalivaLab personnel, will remain open working on research critical to the COVID-19 response; onsite SalivaLab personnel will be able to receive samples, and in some cases, test and send data.  All Salimetrics’ laboratory personnel are following PPE guidelines per CDC Biosafety Level 2 (BSL-2).

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