Salivary Cytokine Collection Protocol
Collection volume, general considerations, and basic guidelines to maximize salivary Interleukin-8 sample integrity. Use this analyte-specific collection protocol to plan you collection methodology and sampling schemes.
3. Technical Summary
|Optimum Collection Volume:||100 μL|
|Assay Range:||0.07 – 2336 pg/mL|
Interleukin-8 (IL-8), also known as neutrophil chemotactic factor, belongs to the CXC chemokine family and mainly serves as an immune mediator of neutrophil-dependent acute inflammation (2). IL-8 is produced in various cells and tissues such as phagocytes and mesenchymal cells exposed to inflammatory stimulus, and primarily targets the recruitment and activation of neutrophil granulocytes and other intra-and extracellular changes in response to infection, inflammation, trauma, environmental stress, and steroid hormones, etc. IL-8 has also been suggested to influence T-cell migration. Although neutrophils are the primary target, a variety of other cells also respond to the presence of IL-8. In serum, IL-8 has shown strong associations with studies in periodontal disease, obesity (3), cancer, oxidative stress (4), rheumatoid arthritis, tumor development and Hepatitis C.
References & Salivary Interleukin-8 Research
- Bickel M. (1993). The role of interleukin-8 in inflammation and mechanisms of regulation. J Periodontol. 64(5 Suppl):456-60.
- Baggiolini M, Clark-Lewis I. (1992). Interleukin-8, a chemotactic and inflammatory cytokine. FEBS Lett. 307(1):97-101.
- Sharabiani MT, et al. (2011). Immunologic profile of excessive body weight. Biomarkers. 16(3):243-51.
- Vlahopoulos S, et al. (1999). Nuclear factor-kappaB-dependent induction of interleukin-8 gene expression by tumor necrosis factor alpha: evidence for an antioxidant sensitive activating pathway distinct from nuclear translocation. Blood. 94(6):1878-89.