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Salivary Androstenedione

Technical Summary

Analyte Summary:
Analyte: ​Androstenedione
Aliases: 4-androstenedione, 4-dione; 4-androstene-3,17-dione
Serum-Saliva Correlation: 0.77
Optimum Collection Volume: 125 μL*
*Add 300 µl to the total volume of all tests for liquid handling
Assay Summary:
Methodology: ELISA
Sensitivity: 5 pg/mL
Assay Range: 10 pg/mL - 2430 pg/mL
Assay Type: Quantitative

Collect Saliva Samples


Better results begin with better saliva collection. This collection protocol features general considerations to maximize salivary Androstenedione analysis. Use this analyte-specific collection protocol to plan your collection methodology and sampling schemes.


Test Saliva Samples

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Salimetrics SalivaLab - Easy & Accurate
Order Code (lab): 5149
Transport Requirements: Ship on Dry Ice
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Add DNA Analysis to My Study

Considerations for adding Salivary DNA to analyte Studies:

You can combine salivary analytes with easy, accurate, and affordable genomic testing using Salimetrics SalivaLab and the same sample that you are already collecting – no specialized saliva collection devices or additional samples are required.

Don’t know what SNPs are right for you? The SalivaLab’s DNA team specializes in genetic testing services, we recommend you Request a DNA Consult (gratis) to learn more about common considerations such as # of samples, participant ethnicity, and IRB Approval.

All DNA Services

DNA Extraction and Normalization
Single Nucleotide Polymorphism (SNP) Genotyping
VNTR & STR Analysis

References & Salivary Androstenedione Research

    1. Yates, J., Deshpande, N. (1974).  Kinetic studies on the enzymes catalyzing the conversion of 17α-hydroxy-progesterone and dehydroepiandrosterone to androstenedione in the human adrenal gland in vitro. J Endocrinol, 6(1), 27-35.
    2. Carson, R.S., Findlay, J.K., Clarke, I.J., Burger, H.G. (1981).  Estradiol, testosterone, and androstenedione in ovine follicular fluid during growth and atresia of ovarian follicles.  Biol Reprod, 24(1), 105-113.
    3. Weinstein, R.L., Kelch, R.P., Jenner, M.R., et al. (1974). Secretion of unconjugated androgens and estrogens by the normal and abnormal human testis before and after human chorionic gonadotropin.  J Clin Invest, 53(1), 1-6.
    4. Dorfman, R. I., Shipley, R. A. (1956). Androgens. New York: John Wiley and Sons.
    5. Labrie, F., Bélanger, A., Cusan, L., Candas, B. (1997).  Physiological changes in dehydroepiandrosterone are not reflected by serum levels of active androgens and estrogens but of their metabolites: Intracrinology.  J Clin Endocrinol Metab, 82(8), 2403-9.
    6. Labrie, F., Luu-The, V. Bélanger, A., et al. (2005).  Is dehydroepiandrosterone a hormone? J Endocrinol, 187(2), 169-96.
    7. Dorn, L.D., Kolko, D.J., Susman, E.J., et al. (2009).  Salivary gonadal and adrenal hormone differences in boys and girls with and without disruptive behavior disorders: Contextual variants.  Biol Psychol, 81(1), 31-39.
    8. King, D.S., Sharp, R.L., Vukovich, M.D., et al. (1999).  Effect of oral androstenedione on serum testosterone and adaptations to resistance training in young men: A randomized controlled trial. JAMA, 281(21), 2020-8.
    9. Leder, B.Z., Longcope, C., Catlin, D.H., et al. (2000).  Oral androstenedione administration and serum testosterone concentrations in young men.  JAMA, 283(6), 779-82.
    10. Leder, B.Z., Leblanc, K.M., Longcope, C., et al. (2002).  Effects of oral androstenedione administration on serum testosterone and estradiol levels in postmenopausal women.  J Clin Endocrinol Metab, 87(12), 5449-54.

    1. Kicman, A.T., Bassindale, T., Cowan, D.A., et al. (2003).  Effect of androstenedione ingestion on plasma testosterone in young women; A dietary supplement with potential health risks. Clin Chem, 49(1), 167-9.
    2. Goldman, J., Wajchenberg, B.L., Liberman, B. et al. (1985).  Contrast analysis for the evaluation of the circadian rhythms of plasma cortisol, androstenedione, and testosterone in normal men and the possible influence of meals.  J Clin Endocrinol Metab, 60(1), 164-67.
    3. Ostrowska, Z. Zwirska-Korczala, K., Pardela, M., et al. (1998).  Circadian variations of androstenedione, dehydroepiandrosterone sulfate and free testosterone in obese women with menstrual disturbances. Endocr Regul, 32(4), 169-76.
    4. Longcope,  C., Baler,  S. (1993).  Androgen and estrogen dynamics: Relationships with age, weight, and menopause status. J Clin Endocrinol Metab, 76(3), 601–4.
    5. Vining, R.F., McGinley, R.A. (1987). The measurement of hormones in saliva: Possibilities and pitfalls.  J Steroid Biochem, 27(1-3), 81-94.

Contact: Salimetrics (USA)
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Salimetrics’ COVID-19 Status – 6 April 2020 – No change to Prior Update:

Salimetrics’ Sales and Customer Service, as well as all other non-laboratory personnel, have successfully transitioned to working remote and are fully able to provide support to our customers.

Our Salimetrics Pennsylvania Manufacturing Center remains open for customer orders and shipments. All Salimetrics’ laboratory personnel are following PPE guidelines per CDC Biosafety Level 2 (BSL-2).

The Salimetrics’ SalivaLab, located in California, is open to receive samples. In accordance with State of California Executive Order N-33-20 effective March 19, 2020, our Carlsbad, California facility, including R&D and SalivaLab personnel, will remain open working on research critical to the COVID-19 response; onsite SalivaLab personnel will be able to receive samples, and in some cases, test and send data.  All Salimetrics’ laboratory personnel are following PPE guidelines per CDC Biosafety Level 2 (BSL-2).



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