It appears that your browser may be outdated and performance may be limited. The My Study builder is best viewed with the latest internet browsers. However, if you’d prefer to use our standard form, you can access it below.

Go to Internet Explorer Quick Quote Form

Dismiss this message

Salivary TNF-Alpha

Technical Summary

Analyte Summary
Analyte: Tumor Necrosis Factor Alpha
Aliases: TNF-Alpha, TNF-α, cachectin
Serum-Saliva Correlation: NA
Optimum Collection Volume: 425 μL*
*Add 300 µl to the total volume of all tests for liquid handling
Assay Summary
Methodology: ELISA/ECL
Sensitivity: 0.106 pg/mL
Assay Range: 0.0314-380 pg/mL
Assay Type: Quantitative

Collect Saliva Samples


Better results begin with better saliva collection. This collection protocol features general considerations to maximize salivary TNF-Alpha analysis. Use this analyte specific collection protocol to plan your collection methodology and sampling schemes.


Test Saliva Samples

@ Salimetrics
Salimetrics SalivaLab - Easy & Accurate
Order Code (lab): 5291
Transport Requirements: Ship on Dry Ice
Related Panels and Profiles: Salivary Cytokine Panel
@ Your Own Lab
Salimetrics Assay Kits - Better Results

The validated method used by Salimetrics is proprietary and not available in assay kit form at this time.

Add DNA Analysis to My Study

Considerations for adding Salivary DNA to analyte Studies:

You can combine salivary analytes with easy, accurate, and affordable genomic testing using Salimetrics SalivaLab and the same sample that you are already collecting – no specialized saliva collection devices or additional samples are required.

Don’t know what SNPs are right for you? The SalivaLab’s DNA team specializes in genetic testing services, we recommend you Request a DNA Consult (gratis) to learn more about common considerations such as # of samples, participant ethnicity, and IRB Approval.

All DNA Services

DNA Extraction and Normalization
Single Nucleotide Polymorphism (SNP) Genotyping
VNTR & STR Analysis

References & Salivary TNF-Alpha Research

      1. Czermak, B.J., Sarma, V., Bless, N.M., et al. (1999).  In vitro and in vivo dependency of chemokine generation on C5a and TNF-α.  J Immunol, 162(4), 2321-25.
      2. Tanabe, K., Matsushima-Nishiwaki, R., Yamaguchi, S., et al. (2010).  Mechanisms of tumor necrosis factor-α-induced interleukin-6 synthesis in glioma cells.  J Neuroinflammation, 7, 16.
      3. Sternberg, E.M. (2006).  Neural regulation of innate immunity: A coordinated nonspecific host response to pathogens.  Nat Rev Immunol, 6(4), 318-28.
      4. Heller, R.A., Krönke, M. (1994).  Tumor necrosis factor receptor-mediated signaling pathways.  J Cell Biol, 126(1), 5-9.)
      5. Mastroeni, P., Ménager, N. (2003).  Development of acquired immunity to Salmonella.  J Med Microbiol, 52(pt 6), 453-59.
      6. Goodsell, D.S. (2006).  The molecular perspective: Tumor necrosis factor.  Oncologist, 11(1), 83-4.
      7. Giannobile, W.V., Beikler, T., Kinney, J.S., et al. (2009). Saliva as a diagnostic tool for periodontal disease: Current state and future directions.  Periodontology 2000, 50, 52-64.
      8. Torre-Amione, G., Kapadia, S., Lee, J., et al. (1996).  Tumor necrosis factor-α and tumor necrosis factor receptors in the failing human heart.  Circulation, 93(4), 704-11.
      9. Kulkarni, K., Selesniemi, K, Brown, T.L. (2006). Interferon-gamma sensitizes the human salivary gland cell line, HSG, to tumor necrosis factor-alpha induced activation of dual apoptotic pathways.  Apoptosis, 11(12), 2205-15.
      10. Wu, A.J., Chen, Z.J., Tsokos, M. et al (1996),  Interferon-gamma induced cell death in a cultured human salivary gland cell line. J Cell Physiol, 167(2), 297-304.
      1. Fernandes, J.C., Martel-Pelletier, J., Pelletier, J.P. (2002). The role of cytokines in osteoarthritis pathophysiology.  Biorheology, 39(1-2), 237-46.
      2. Sun, D., Emmert-Buck, M.R., Fox, P.C. (1998).  Differential cytokine mRNA expression in human labial minor salivary glands in primary Sjögren’s syndrome.  Autoimmunity, 28(3), 125-37.
      3. Fox, R.I., Kang, H.I, Ando, D., et al. (1994).  Cytokine mRNA expression in salivary gland biopsies of Sjögren’s syndrome.  J Immunol, 152(11), 5532-39.

Contact: Salimetrics (USA)
View All International Distributors

Salimetrics’ COVID-19 Status – 8 April 2020 – No change to Prior Update:

Salimetrics’ Sales and Customer Service, as well as all other non-laboratory personnel, have successfully transitioned to working remote and are fully able to provide support to our customers.

Our Salimetrics Pennsylvania Manufacturing Center remains open for customer orders and shipments. All Salimetrics’ laboratory personnel are following PPE guidelines per CDC Biosafety Level 2 (BSL-2).

The Salimetrics’ SalivaLab, located in California, is open to receive samples. In accordance with State of California Executive Order N-33-20 effective March 19, 2020, our Carlsbad, California facility, including R&D and SalivaLab personnel, will remain open working on research critical to the COVID-19 response; onsite SalivaLab personnel will be able to receive samples, and in some cases, test and send data.  All Salimetrics’ laboratory personnel are following PPE guidelines per CDC Biosafety Level 2 (BSL-2).



COVID-19 Notice