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Melatonin onset as a biomarker for the analysis of circadian rhythm, for chronobiological disorders and obstructive sleep-wake phase shifts.
With the increased clinical focus on sleep as it relates to many aspects of human health and performance, “Dim Light Melatonin Onset” (DLMO) continues to serve as a pivotal tool to assess circadian rhythm sleep disorders and associated health concerns. DLMO, the time at which melatonin levels rise above daytime baseline levels, can reliably be assessed using saliva, as salivary melatonin concentrations are highly correlated with levels in the blood. Frequent saliva sampling for DLMO estimation can be performed in the comfort of one’s home and does need an overnight stay at a sleep lab. Determining DLMO is relevant in discriminating circadian rhythm related sleep issues from other non-circadian related sleep disorders, and it is also important in establishing the timing of exogenous melatonin administration when treating delayed phase sleep disorders. Sleep functions and circadian timing are also known to contribute to diseases such as cancer, heart disease, obesity, and diabetes, and researchers are increasingly focused on elucidating the role of phase shift disorders and their impact on these diseases.
Avoid foods such as pitted fruit, bananas and chocolate 24 hours before sample collection.
About Salivary DLMO
Salivary Melatonin measures in the form of a DLMO profile have been shown to be a reliable method to assess endogenous circadian rhythms. While the hormone melatonin continues to play a critical role in determining an individual’s sleep-wake rhythm, the timing of melatonin onset has been shown to strongly correlate with sleep onset and continuity. Therefore, to fully assess phase shifted melatonin rhythms or disturbances in the sleep-wake cycle, it is often necessary to understand individual circadian rhythms. To accurately perform this assessment, researchers commonly utilize salivary melatonin as a marker of dim light melatonin onset or a salivary DLMO profile as the preferred method for many sleep-wake disorder studies. Salivary melatonin profiles now provide a personalized window of circadian timing that effectively enables researchers to determine circadian phase position and study the influence of circadian timing.
Better results begin with better saliva collection. This collection protocol features general considerations to maximize salivary DLMO analysis. Use this collection protocol to plan your collection methodology and sampling schemes.
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Salimetrics’ COVID-19 Status – 6 April 2020 – No change to Prior Update:
Salimetrics’ Sales and Customer Service, as well as all other non-laboratory personnel, have successfully transitioned to working remote and are fully able to provide support to our customers.
Our Salimetrics Pennsylvania Manufacturing Center remains open for customer orders and shipments. All Salimetrics’ laboratory personnel are following PPE guidelines per CDC Biosafety Level 2 (BSL-2).
The Salimetrics’ SalivaLab, located in California, is open to receive samples. In accordance with State of California Executive Order N-33-20 effective March 19, 2020, our Carlsbad, California facility, including R&D and SalivaLab personnel, will remain open working on research critical to the COVID-19 response; onsite SalivaLab personnel will be able to receive samples, and in some cases, test and send data. All Salimetrics’ laboratory personnel are following PPE guidelines per CDC Biosafety Level 2 (BSL-2).