*Available as part of the Salimetrics Cytokine Panel - Not available as an individual test*Human interleukin-10 (IL-10). IL-10 is mainly produced by monocytes and some lymphocytes as a 20.5 kDa, glycosylated homodimeric protein. It is an anti-inflammatory cytokine that inhibits the synthesis of numerous cytokines (including IFN-γ, IL-2, IL-3, TNF-α, TNF-β, and GM-CSF) that suppress Th1 proinflammatory responses involved in promoting phagocytosis. Secretion is at very low levels in unstimulated tissues and IL-10 regulates its own expression in a negative feedback loop. IL-10 also functions to enhance B lymphocyte survival, proliferation and antibody production. Like IL-6, IL-10 is linked to the myokines, as exercise provokes an increase in circulating levels of IL-1ra, IL-10, and sTNF-R, suggesting that physical exercise fosters an environment of anti-inflammatory cytokines. IL-10 levels are moderately elevated during acute stress (1) and oral cancer (2).
|Aliases:||IL-10, human cytokine synthesis inhibitory factor (CSIF)|
|Optimum Collection Volume:||100 μL|
|Assay Range:||0.04–233 pg/mL|
Collecting Saliva for Cytokines
CYTOKINE PANEL SALIVA COLLECTION CONSIDERATIONS
Better results begin with better saliva collection. This collection protocol features general considerations to maximize salivary cytokine analysis. Use this analyte specific collection protocol to plan your collection methodology and sampling schemes.
Test Samples at Salimetrics
|Order Code (lab):||5209.04|
|Transport Requirements:||Ship on Dry Ice|
|Related Panels and Profiles:||Salivary Cytokine Panel|
The validated method used by Salimetrics is proprietary and not available in assay kit form at this time.
Add DNA Analysis to My Study
Considerations for adding Salivary DNA to analyte Studies:
You can combine salivary analytes with easy, accurate, and affordable genomic testing using Salimetrics SalivaLab and the same sample that you are already collecting – no specialized saliva collection devices or additional samples are required.
Don’t know what SNPs are right for you? The SalivaLab’s DNA team specializes in genetic testing services, we recommend you Request a DNA Consult (gratis) to learn more about common considerations such as # of samples, participant ethnicity, and IRB Approval.
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References & Salivary Interleukin-10 Research
- Szabo YZ, Newton TL, Miller JJ, Lyle KB, Fernandez-Botran R. Acute stress induces increases in salivary IL-10 levels. Stress. 2016;19(5):499-505.
- Aziz S, Ahmed SS, Ali A, Khan FA, Zulfiqar G, Iqbal J, et al. Salivary Immunosuppressive Cytokines IL-10 and IL-13 Are Significantly Elevated in Oral Squamous Cell Carcinoma Patients. Cancer Invest. 2015;33(7):318-28.
- Rajendran P, Chen YF, Chen YF, Chung LC, Tamilselvi S, Shen CY, et al. The multifaceted link between inflammation and human diseases. Journal of cellular physiology. 2018;233(9):6458-71.
- Val M, Sidoti Pinto GA, Manini L, Gandolfo S, Pentenero M. Variations of salivary concentration of cytokines and chemokines in presence of oral squamous cell carcinoma. A case-crossover longitudinal prospective study. Cytokine. 2019;120:62-5.
- Wang X, Kaczor-Urbanowicz KE, Wong DT. Salivary biomarkers in cancer detection. Med Oncol. 2017;34(1):7.
- Huck O, Buduneli N, Bravo D. Inflammatory Mediators in Periodontal Pathogenesis. Mediators Inflamm. 2019;2019:2610184.
- Silva N, Abusleme L, Bravo D, Dutzan N, Garcia-Sesnich J, Vernal R, et al. Host response mechanisms in periodontal diseases. J Appl Oral Sci. 2015;23(3):329-55.
- Gaba FI, Sheth CC, Veses V. Salivary biomarkers and their efficacies as diagnostic tools for Oral Squamous Cell Carcinoma: Systematic review and meta-analysis. J Oral Pathol Med. 2018.
- Slavish DC, Graham-Engeland JE, Smyth JM, Engeland CG. Salivary markers of inflammation in response to acute stress. Brain, behavior, and immunity. 2015;44:253-69.
- Sheth CC, Lopez-Pedrajas RM, Jovani-Sancho MDM, Gonzalez-Martinez R, Veses V. Modulation of salivary cytokines in response to alcohol, tobacco and caffeine consumption: a pilot study. Sci Rep. 2018;8(1):16687.