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Salivary Interleukin-10

Salivary Cytokines Quick Start Research Guide

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Biomarkers

1. How to collect Salivary Interleukin-10

APPROVED SALIVARY CYTOKINE COLLECTION METHODS

Passive Drool Saliva Collection Kit

SalivaBio Passive Drool Method

Use With: Adults, Children 6+

Salivary Cytokine Collection Protocol

Collection volume, general considerations, and basic guidelines to maximize salivary IL-10 sample integrity. Use this analyte-specific collection protocol to plan you collection methodology and sampling schemes.

Biomarkers

2. How to Assay for Salivary Interleukin-10

Easy and accurate results from the most trusted Salivary Bioscience Laboratory.

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Order Code5209

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3. Technical Summary

Analyte Summary
Analyte: Interleukin-10
Aliases: IL-10, human cytokine synthesis inhibitory factor (CSIF)
Serum-Saliva Correlation: NA
Optimum Collection Volume: 100 μL
Assay Summary
Methodology: ECL
Sensitivity: 0.04 pg/mL
Assay Range: 0.04 – 1420 pg/mL
Assay Type: Quantitative

Background

Human interleukin-10 (IL-10). IL-10 is mainly produced by monocytes and some lymphocytes as a 20.5 kDa, glycosylated homodimeric protein. It is an anti-inflammatory cytokine that inhibits the synthesis of numerous cytokines (including IFN-γ, IL-2, IL-3, TNF-α, TNF-β, and GM-CSF) that suppress Th1 proinflammatory responses involved in promoting phagocytosis. Secretion is at very low levels in unstimulated tissues and IL-10 regulates its own expression in a negative feedback loop. IL-10 also functions to enhance B lymphocyte survival, proliferation and antibody production. Like IL-6, IL-10 is linked to the myokines, as exercise provokes an increase in circulating levels of IL-1ra, IL-10, and sTNF-R, suggesting that physical exercise fosters an environment of anti-inflammatory cytokines. IL-10 levels are moderately elevated during acute stress (1) and oral cancer (2).

References & Salivary Interleukin-10 Research

  1. Szabo YZ, Newton TL, Miller JJ, Lyle KB, Fernandez-Botran R. Acute stress induces increases in salivary IL-10 levels. Stress. 2016;19(5):499-505.
  2. Aziz S, Ahmed SS, Ali A, Khan FA, Zulfiqar G, Iqbal J, et al. Salivary Immunosuppressive Cytokines IL-10 and IL-13 Are Significantly Elevated in Oral Squamous Cell Carcinoma Patients. Cancer Invest. 2015;33(7):318-28.
  3. Rajendran P, Chen YF, Chen YF, Chung LC, Tamilselvi S, Shen CY, et al. The multifaceted link between inflammation and human diseases. Journal of cellular physiology. 2018;233(9):6458-71.
  4. Val M, Sidoti Pinto GA, Manini L, Gandolfo S, Pentenero M. Variations of salivary concentration of cytokines and chemokines in presence of oral squamous cell carcinoma. A case-crossover longitudinal prospective study. Cytokine. 2019;120:62-5.
  5. Wang X, Kaczor-Urbanowicz KE, Wong DT. Salivary biomarkers in cancer detection. Med Oncol. 2017;34(1):7.
  6. Huck O, Buduneli N, Bravo D. Inflammatory Mediators in Periodontal Pathogenesis. Mediators Inflamm. 2019;2019:2610184.
  7. Silva N, Abusleme L, Bravo D, Dutzan N, Garcia-Sesnich J, Vernal R, et al. Host response mechanisms in periodontal diseases. J Appl Oral Sci. 2015;23(3):329-55.
  8. Gaba FI, Sheth CC, Veses V. Salivary biomarkers and their efficacies as diagnostic tools for Oral Squamous Cell Carcinoma: Systematic review and meta-analysis. J Oral Pathol Med. 2018.
  9. Slavish DC, Graham-Engeland JE, Smyth JM, Engeland CG. Salivary markers of inflammation in response to acute stress. Brain, behavior, and immunity. 2015;44:253-69.
  10. Sheth CC, Lopez-Pedrajas RM, Jovani-Sancho MDM, Gonzalez-Martinez R, Veses V. Modulation of salivary cytokines in response to alcohol, tobacco and caffeine consumption: a pilot study. Sci Rep. 2018;8(1):16687.

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