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When nicotine from tobacco smoke is taken into the lungs and enters the bloodstream, the principle metabolite produced in the liver is cotinine. Cotinine diffuses easily from blood into saliva, and salivary cotinine and blood levels are highly correlated. (1) Cotinine in saliva has a longer half-life than nicotine (more than 10 hours), and the literature has documented it to be a specific and sensitive marker for determining exposure to tobacco and nicotine, allowing for primary and second-hand exposure determination. (2,3,4)
*Add 300 µl to the total volume of all tests for liquid handling
0.8 ng/mL - 200 ng/mL
Salivary Cotinine Example Ranges*
Standard Deviation (ng/mL)
*Note: Examples are to be used as a guide only. Each laboratory should estabilsh its own range.
Collect Saliva Samples
COTININE SALIVA COLLECTION CONSIDERATIONS
Better results begin with better saliva collection. This collection protocol features general considerations to maximize salivary cotinine analysis. Use this analyte-specific collection protocol to plan your collection methodology and sampling schemes.
Considerations for adding Salivary DNA to analyte Studies:
You can combine salivary analytes with easy, accurate, and affordable genomic testing using Salimetrics SalivaLab and the same sample that you are already collecting – no specialized saliva collection devices or additional samples are required.
Don’t know what SNPs are right for you? The SalivaLab’s DNA team specializes in genetic testing services, we recommend you Request a DNA Consult (gratis) to learn more about common considerations such as # of samples, participant ethnicity, and IRB Approval.
Van Vunakis, H., Tashkin, D.P., Rigas, B., et al. (1989). Relative sensitivity and specificity of salivary and serum cotinine in identifying tobacco-smoking status of self-reported non-smokers and smokers of tobacco and/or marijuana. Arch Environ Health, 44(1), 53-58.
Watts, R.R., Longone, J.J., Kinght, G.J., & Lewtas, J. (1990). Cotinine analytical workshop report: Consideration of analytical methods for determining cotinine in human body fluids as a measure of passive exposure to tobacco smoke. Environ Health Perspect, 84, 173-82.
Roche, D., Callai, F., Reungoat, P., & Momas, I. (2001). Adaptation of an enzyme immunoassay to assess urinary cotinine in nonsmokers exposed to tobacco smoke. Clin Chem, 47(5), 950-52.
Chard, T. (1990). An introduction to radioimmunoassay and related techniques (4th ed.). Amsterdam: Elsevier.
Granger, D.A., Blair, C., Willoughby, M., Kivlighan, K.T., Hibel, L.C., Fortunato, C.K., Wiegand, L.E., & The Family Life Project Investigators (2007). Individual differences in salivary cortisol and alpha-amylase in mothers and infants: Relation to tobacco smoke exposure. Dev Psychobiol, 49(7), 692-701.
Safe Saliva Collection in the COVID-19 Era
We have Good News!
Successfully recalibrate your saliva collection and handling procedures with these new safety and compliance guidelines for the COVID-19 era. These guidelines are good practice, easy to follow, and will allow you to begin or continue your research during this historical period of stress and unease… (Read More)