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Progesterone Saliva Collection

Approved Collection Methods

Passive Drool

Special Considerations

Consider documenting use of progesterone containing contraceptives.

Progesterone may not be detectable in the saliva of males or females prior to the onset of puberty.

Consider recording the menstrual cycle day count or equivalent.

This analyte is sensitive to freeze-thaw degradation. Sample collection, storage, and handling should be carefully designed to minimize the impact of freeze-thaw cycles.

Consider documenting parameters to estimate saliva flow-rate (ie; time taken to collect and sample volume). Consistency in collection method is recommended to avoid introducing unsystematic error into your study data.

Saliva Collection Protocol

Before Sample Collection

  • Avoid foods with high sugar, acidity, or caffeine immediately before sample collection.
  • Document consumption of alcohol, caffeine, nicotine, and prescription/over-the-counter medications within the prior 12 hours.
  • Document vigorous physical activity and the presence of oral disease, injury or inflammation.
  • Do not brush teeth or eat a major meal within 60 minutes of sample collection.
  • Rinse mouth with water to remove food residue and then wait at least 10 minutes before collecting saliva.

During Sample Collection

  • Recommended Collection Volume: 125 µl*
  • Use a collection device that has been validated for the measurement of this analyte.
  • Follow your selected sample collection device/method protocol.

*Add 300 µl to the total volume of all tests for liquid handling

After Sample Collection

  • Record the time and date of specimen collection.
  • Refrigerate samples immediately (if possible) and freeze at or below -20°C (household freezer) as soon as possible (within 6 hours of sample collection)
  • Samples visibly contaminated with blood should be recollected.
  • Do not add preservatives to saliva samples unless it has been previously validated with the assay.
  • Consider aliqouting samples to avoid multiple freeze-thaws.
Contact: Salimetrics (USA)
View All International Distributors

Salimetrics’ COVID-19 Status – 8 April 2020 – No change to Prior Update:

Salimetrics’ Sales and Customer Service, as well as all other non-laboratory personnel, have successfully transitioned to working remote and are fully able to provide support to our customers.

Our Salimetrics Pennsylvania Manufacturing Center remains open for customer orders and shipments. All Salimetrics’ laboratory personnel are following PPE guidelines per CDC Biosafety Level 2 (BSL-2).

The Salimetrics’ SalivaLab, located in California, is open to receive samples. In accordance with State of California Executive Order N-33-20 effective March 19, 2020, our Carlsbad, California facility, including R&D and SalivaLab personnel, will remain open working on research critical to the COVID-19 response; onsite SalivaLab personnel will be able to receive samples, and in some cases, test and send data.  All Salimetrics’ laboratory personnel are following PPE guidelines per CDC Biosafety Level 2 (BSL-2).

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