Salivary Cytokine Collection Protocol
Collection volume, general considerations, and basic guidelines to maximize salivary IL-12p70 sample integrity. Use this analyte-specific collection protocol to plan you collection methodology and sampling schemes.
3. Technical Summary
|Aliases:||IL-12p70, Interleukin-12 subunit p70|
|Optimum Collection Volume:||100 μL|
|Assay Range:||0.11 – 2180 pg/mL|
Human interleukin-12p70 (IL-12p70) activates T cells and natural killer cells that stimulate the production of IFN-γ. It is involved in a number of disorders including periodontitis (1) and chronic toxoplasmosis (2) among others. IL-12p70 is a disulfide-linked heterodimer consisting of IL-12a (p35 subunit) and IL-12b (p40 subunit), mainly produced by macrophages and T lymphocytes and is part of a larger cytokine family that are attractive targets for therapeutic intervention (3,4). The IL-12a subunit also dimerizes with Ebi3 to form IL-35, and the IL-12b subunit dimerizes with IL-23a to form IL-23. IL-12, IL-23, IL-27, and IL-35 are all heterodimeric proteins and members of the IL-12 cytokine family (4).
References & Salivary IL-12p70 Research
- Issaranggun Na Ayuthaya B, Everts V, Pavasant P. The immunopathogenic and immunomodulatory effects of interleukin-12 in periodontal disease. European journal of oral sciences. 2018;126(2):75-83.
- Zundler S, Neurath MF. Interleukin-12: Functional activities and implications for disease. Cytokine Growth Factor Rev. 2015;26(5):559-68.
- Teng MW, Bowman EP, McElwee JJ, Smyth MJ, Casanova JL, Cooper AM, et al. IL-12 and IL-23 cytokines: from discovery to targeted therapies for immune-mediated inflammatory diseases. Nat Med. 2015;21(7):719-29.
- Tait Wojno ED, Hunter CA, Stumhofer JS. The Immunobiology of the Interleukin-12 Family: Room for Discovery. Immunity. 2019;50(4):851-70.
- Rajendran P, Chen YF, Chen YF, Chung LC, Tamilselvi S, Shen CY, et al. The multifaceted link between inflammation and human diseases. Journal of cellular physiology. 2018;233(9):6458-71.
- Val M, Sidoti Pinto GA, Manini L, Gandolfo S, Pentenero M. Variations of salivary concentration of cytokines and chemokines in presence of oral squamous cell carcinoma. A case-crossover longitudinal prospective study. Cytokine. 2019;120:62-5.
- Wang X, Kaczor-Urbanowicz KE, Wong DT. Salivary biomarkers in cancer detection. Med Oncol. 2017;34(1):7.
- Huck O, Buduneli N, Bravo D. Inflammatory Mediators in Periodontal Pathogenesis. Mediators Inflamm. 2019;2019:2610184.
- Silva N, Abusleme L, Bravo D, Dutzan N, Garcia-Sesnich J, Vernal R, et al. Host response mechanisms in periodontal diseases. J Appl Oral Sci. 2015;23(3):329-55.
- Gaba FI, Sheth CC, Veses V. Salivary biomarkers and their efficacies as diagnostic tools for Oral Squamous Cell Carcinoma: Systematic review and meta-analysis. J Oral Pathol Med. 2018.
- Slavish DC, Graham-Engeland JE, Smyth JM, Engeland CG. Salivary markers of inflammation in response to acute stress. Brain, behavior, and immunity. 2015;44:253-69.
- Sheth CC, Lopez-Pedrajas RM, Jovani-Sancho MDM, Gonzalez-Martinez R, Veses V. Modulation of salivary cytokines in response to alcohol, tobacco and caffeine consumption: a pilot study. Sci Rep. 2018;8(1):16687.