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1. How to collect Salivary Estrone
APPROVED SALIVARY ESTRONE COLLECTION METHODS
Salivary Estrone Collection Protocol
Collection volume, general considerations, and basic guidelines to maximize salivary estrone sample integrity. Use this analyte-specific collection protocol to plan you collection methodology and sampling schemes.
2. How to Assay for Salivary Estrone
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Salivary Estrone ELISA Kit
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Salimetrics Assay #1-3202
The Salimetrics Salivary Estrone (E1) Enzyme Immunoassay Kit was specifically designed to standardize the detection of estrone in saliva samples for research and biomedical laboratories. Using a small sample volume, this assay kit has an extended range that spans the expected estrone levels found in human saliva. The average inter- and intra-assay precision coefficients of variation are low with no deleterious matrix effects often found in saliva which are characterized through dilution- and spike-recovery validation procedures. This estrone assay kit has also been formatted to minimize cross reactivity for related steroids. Read More...| Assay Protocol |
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Rev. 04.19.19
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| Specifications | |
|---|---|
| Catalog#: | 1-3202 |
| Regulatory Status: | RUO |
| Format: | 96-well plate |
| Assay Time: | ~ 4 hrs |
| Sample Volume/Test: | 100 µL |
| Sensitivity: | 1.4 pg/mL |
| Assay Range: | 3.1 pg/mL - 300 pg/mL |
| Storage Requirements: | 2-8°C |
| Tests Per Kit | |
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| Singlet: | 76 |
| Duplicate: | 38 |
| Target Analyte |
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Technical Documentation
Assay Kit Overview
Intended Use
The Salimetrics Estrone Enzyme Immunoassay Kit is a competitive immunoassay specifically designed and validated for the quantitative measurement of salivary Estrone. It is not intended for diagnostic use. It is intended only for research use in humans and some animals. Salimetrics has not validated this kit for serum or plasma samples.
Introduction
Estrone [3-hydroxy-1,3,5(10)-estratrien-17-one; E1] is a naturally occurring steroidal hormone. A major portion of Estrone is produced from androstenedione in prepubertal children, men, and postmenopausal women. Circulating Estrone levels are relatively high at birth in both males and females, decrease postnatally, and increase during puberty. Of the three major estrogens, Estrone is predominant after menopause in women. Estrone is primarily secreted by the ovaries in premenopausal women, peaking in the preovulatory phase with a smaller secondary increase during the luteal phase. Estrone is a primary component of many pharmaceutical preparations. Research concerning Estrone is often focused on pregnancy, reproduction, and menopause. However, estrogens affect a diverse group of biological processes such as arterial vasodilation, bone density, cognitive function, and neuroprotection. Estrogens are also studied in regard to coronary artery disease, immunocompetence, cancer susceptibility and polycystic ovarian syndrome.
Salivary Estrone Assay Principle
This is a competitive immunoassay kit. Estrone in standards and samples compete with Estrone conjugated to horseradish peroxidase for the antibody binding sites on a microtitre plate. After incubation, unbound components are washed away. Bound Estrone Enzyme Conjugate is measured by the reaction of the horseradish peroxidase enzyme to the substrate tetramethylbenzidine (TMB). This reaction produces a blue color. A yellow color is formed after stopping the reaction with an acidic solution. The optical density is read on a standard plate reader at 450 nm. The amount of Estrone Enzyme Conjugate detected is inversely proportional to the amount of Estrone present in the sample.
3. Technical Summary
| Analyte Summary | |
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| Analyte: | Estrone |
| Aliases: | 3-hydroxy-1,3,5(10)-estratrien-17-one, E1 |
| Serum-Saliva Correlation: | NA |
| *Optimum Collection Volume: | 255 μL |
| Assay Summary | |
|---|---|
| Methodology: | ELISA |
| Sensitivity: | 1.4 pg/mL |
| Assay Range: | 3.1 pg/mL - 300 pg/mL |
| Assay Type: | Quantitative |
Background
Estrone (3-hydroxy-1,3,5(10)-estratrien-17-one; E1) is one of the three main estrogenic steroid hormones produced in humans. Circulating estrone levels are relatively high at birth in both males and females, decrease postnatally, and increase during puberty. (1) Of the three major estrogens, estrone is predominant after menopause in women. (1) In premenopausal women estrone is primarily produced by conversion of androstenedione in the ovaries, with concentrations peaking in the preovulatory phase and a smaller secondary increase during the luteal phase. (1,2) In post-menopausal women, children, and men, estrone is largely produced by conversion of androstenedione in peripheral tissues. (1,2,3) Estrone is subsequently reduced to estradiol in various peripheral tissues. (3) In non-pregnant women, only about 3% of estrone in the bloodstream is not bound to proteins. (4) Unbound estrone enters saliva from blood via intracellular mechanisms, and in saliva the majority of the estrone remains unbound to protein. The saliva and plasma values are highly correlated. (5)
References & Salivary Estrone Research
- Faiman, C., Winter, J.S., Reyes, F.I. (1976). Patterns of gonadotrophins and gonadal steroids throughout life. Clin Obstet Gynecol, 3(3), 467-83.
- Cañez, M.S., Lee, K.J., & Olive, D.L. (1992). Progestogens and estrogens. Infert Reprod Med Clin North Amer, 3, 59-78.
- Sasano, H., Harada, N. (1998). Intratumoral aromatase in human breast, endometrial, and ovarian malignancies. Endocr Rev, 19(5), 593-607.
- Speight, A.C., Hancock, K.W., Oakey, R.E. (1979). Non-protein-bound oestrogens in plasma and urinary excretion of unconjugated oestrogens in non-pregnant women. J Endocrinol, 83, 385-91.
- Vining, R.F., McGinley, R.A. (1987). The measurement of hormones in saliva: Possibilities and pitfalls. J Steroid Biochem, 27(1-3), 81-94.