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Archives: Analytes

FTO SNP Genotyping

Salivary DNA Analysis

Genotyping creates an inter-individual biological profile that enhances the conclusions of observed abnormalities in participant groups. The SalivaLab’s DNA isolation protocol yields high purity, low salt DNA with A260/A280 ratios (indicating protein content) consistently at or above 1.8 and the A260/A230 ratios at or above 1.5. These high quality results with low sample retest rates […]

SARS-CoV-2 (N) IgG Saliva Collection

Print Approved Collection Methods Passive Drool SalivaBio Swabs Special Considerations Consistency in collection methods and sample handling is recommended for optimal results. Production of volumes larger than 1 mL may produce a diluted sample that may necessitate a recollection. Saliva Collection Protocol Before Sample Collection Avoid foods with high sugar or acidity immediately before sample [...]
COVID-19 in Saliva

Salivary SARS-CoV-2 (N) IgG

There is an urgent need to better understand COVID-19 antibody levels and duration of protective immunity for public health considerations. Coronavirus antibody testing in saliva has shown utility as a sensitive and specific serum alternative for large scale immune-surveillance studies (1). Importantly, IgG in saliva is derived from serum, so specificity and reactivity of salivary […]

Salivary IgM

Immunoglobulin M, or IgM, is present in two major forms in the circulation referred to as natural or adaptive (1). Natural IgMs are low affinity pentameric molecules that bind to invading pathogens without requiring prior exposure (2). They act to directly neutralize viruses, activate complement, trigger phagocytosis and drive antibody dependent cell cytotoxicity (3). Adaptive […]

Total IgM Saliva Collection

Print Approved Collection Methods Passive Drool SalivaBio Swabs Special Considerations If collecting with swab devices, it is important to know that concentrations of this analyte may vary depending on the location in the mouth; consistency in swab placement is important. This analyte is sensitive to freeze-thaw degradation. Sample collection, storage, and handling should be carefully [...]
IL-17A in Saliva

Salivary ​Interleukin-17A

Human Interleukin-17A (IL-17A), is a disulfide linked glycoprotein that is secreted as a 17.5 kDa homodimer mainly by activated T-helper 17 (Th17) cells (1, 2). IL-17A is an important regulator of inflammation whose signaling induces pro-inflammatory cytokines and chemokines that recruit cells of the innate immune system to sites of infection (2). IL-17A also induces […]

IL-7 in Saliva

Salivary ​Interleukin-7

Human Interleukin 7 (IL-7) activity is important for every stage of T cell development and survival of naïve T cells and generation and maintenance of CD4 and CD8 T cell memory. It is also important for the formation of lymphoid structure and defense mechanisms mediated by lymphoid cells. Pathologies associated with IL-7 are those mediated […]

IL-5 in Saliva

Salivary ​Interleukin-5

Human Interleukin 5 (IL-5), mainly drives the maturation and maintenance of eosinophils and antibody secreting B cells and is a glycosylated 15.2 kDa disulfide linked homodimer mainly produced by eosiniophils (1) and T helper type 2 (Th2) cells (2). Dysregulation of IL-5 is associated with eosinophilia, atopic dermatitis and pulmonary fibrosis. IL-5 is also expressed […]

Total IgG Saliva Collection

Print Approved Collection Methods Passive Drool SalivaBio Swabs Special Considerations If collecting with swab devices, it is important to know that concentrations of this analyte may vary depending on the location in the mouth; consistency in swab placement is important. This analyte is sensitive to freeze-thaw degradation. Sample collection, storage, and handling should be carefully [...]
Salivary Total IgG

Salivary IgG

Immunoglobulin G (IgG) is critical for host immune-defense against infectious pathogens and is the most abundant antibody found in blood. Within the IgG class, there are four subclasses (IgG1, IgG2, IgG3 and IgG4). The term total IgG collectively refers to all IgG subclasses all of which have varying unique reactivities. In most cases an immune […]

Osteocalcin Saliva Collection

Print Approved Collection Methods Passive Drool SalivaBio Swabs Special Considerations Consider documenting parameters to estimate saliva flow-rate (ie; time taken to collect and sample volume). Consistency in collection method is recommended to avoid introducing unsystematic error into your study data. Saliva Collection Protocol Before Sample Collection Avoid foods with high sugar, acidity, or caffeine immediately [...]
Osteocalcin in Saliva

Salivary Osteocalcin

Osteocalcin (also known as OCN, OC, and Bone-Gla-Protein (BGP) or bone gamma-carboxyglutamic acid-containing protein (BGLAP)), is a bone-derived hormone protein released solely by osteoblasts during bone remodeling in vertebrates. Research interest in Osteocalcin has revealed endocrine-related properties related to several physiological pathways. Recent findings suggest that Osteocalcin may also be a key factor in the […]

IL-13 in Saliva

Salivary Interleukin-13

Human interleukin-13 (IL-13) is secreted by a variety of immune cells as a 15.8 kDa, glycosylated monomeric cytokine involved in a number of biological processes. It stimulates B-cell proliferation, macrophage activation, immunoglobulin production, protein secretion, and phosphorylation of Stat6 protein. IL-13 is a central regulator of IgE synthesis, mucus hypersecretion and a mediator of allergic […]

IL-12p70 in Saliva

Salivary Interleukin-12p70

Human interleukin-12p70 (IL-12p70) activates T cells and natural killer cells that stimulate the production of IFN-γ. It is involved in a number of disorders including periodontitis (1) and chronic toxoplasmosis (2) among others. IL-12p70 is a disulfide-linked heterodimer consisting of IL-12a (p35 subunit) and IL-12b (p40 subunit), mainly produced by macrophages and T lymphocytes and […]

IL-10 in Saliva

Salivary Interleukin-10

Human interleukin-10 (IL-10). IL-10 is mainly produced by monocytes and some lymphocytes as a 20.5 kDa, glycosylated homodimeric protein. It is an anti-inflammatory cytokine that inhibits the synthesis of numerous cytokines (including IFN-γ, IL-2, IL-3, TNF-α, TNF-β, and GM-CSF) that suppress Th1 proinflammatory responses involved in promoting phagocytosis. Secretion is at very low levels in […]

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