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Diagnostic Salivary Cotinine ELISA Kit (FDA, CE Mark)


Technical Summary

Assay Protocol
Catalog#: 1-2112
Regulatory Status: 510(k) Exempt, CE Mark
Format: 96-well plate
Assay Time: ~ 2.5 hrs
Sample Volume/Test: 20 µL
Sensitivity: 0.15 ng/mL
Assay Range: 0.8 ng/mL - 200 ng/mL
Storage Requirements: 2-8°C
Tests Per Kit
Singlet: 76
Duplicate: 38
Target Analyte

References & Salivary Cotinine Research

  1. Benowitz, N.L. (1996). Cotinine as a biomarker of environmental tobacco smoke exposure.  Epidemiol Rev, 18(2), 188-204.
  2. Dhar, P. (2004). Measuring tobacco smoke exposure: Quantifying nicotine/cotinine concentration in biological samples by colorimetry, chromatography and immunoassay methods. J Pharm Biomed Anal, 35(1), 155- 68.
  3. Alterman, A.I., Gariti, P., Niedbala, R.S. (2002). Varying results for immunoassay screening kits for cotinine levels. Psychol Addict Behav, 16(3), 256-59.
  4. Van Vunakis, H., Tashkin, D.P., Rigas, B., et al. (1989). Relative sensitivity and specificity of salivary and serum cotinine in identifying tobacco-smoking status of self-reported non-smokers and smokers of tobacco and/or marijuana. Arch Environ  Health, 44(1), 53-58.
  5. Benowitz, N.L., Hukkanen, J., & Jacob, P., 3rd (2009). Nicotine chemistry, metabolism, kinetics and biomarkers.  Handb Exp Pharmacol, 192, 29-60.
  6. Watts, R.R., Longone, J.J., Kinght, G.J., & Lewtas, J. (1990). Cotinine analytical workshop report: Consideration of analytical methods for determining cotinine in human body fluids as a measure of passive exposure to tobacco smoke.  Environ Health Perspect, 84, 173-82.
  7. Roche, D., Callai, F., Reungoat, P., & Momas, I. (2001). Adaptation of an enzyme immunoassay to assess urinary cotinine in nonsmokers exposed to tobacco smoke.  Clin Chem, 47(5), 950-52.
  8. Chard, T. (1990). An introduction to radioimmunoassay and related techniques (4th ed.).  Amsterdam: Elsevier.
  9. Granger, D.A., Blair, C., Willoughby, M., Kivlighan, K.T., Hibel, L.C., Fortunato, C.K., Wiegand, L.E., & The Family Life Project Investigators (2007). Individual differences in salivary cortisol and alpha-amylase in mothers and infants: Relation to tobacco smoke exposure.  Dev Psychobiol, 49(7), 692-701.
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