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Diagnostic Salivary Cotinine ELISA Kit (FDA, CE Mark)
Salimetrics Assay #1-2112 (in vitro diagnostic use)
Salimetrics Salivary Cotinine ELISA provides researchers with a sensitive method to objectively quantify differences in individual cotinine levels (nicotine exposure), using an easy to collect, non-invasive saliva-sample. Validated for clinical use, and highly correlated with serum levels, Salimetrics Cotinine assay’s high-sensitivity distinguishes between active smokers, non-smokers, and recent second-hand smoke exposure. Cotinine, the most common biomarker for nicotine exposure analysis, and is primarily measured in smoking cessation studies, e-cigarette and public health research, and to assess second-hand smoke exposure in children. Companies may also evaluate salivary cotinine levels for tobacco use relative to employment, health, and life-insurance purposes. Cotinine is a preferred biomarker to evaluate tobacco use, because it maintains a half-life of up to 16-hours, as opposed to nicotine’s half-life of just several hours.
Van Vunakis, H., Tashkin, D.P., Rigas, B., et al. (1989). Relative sensitivity and specificity of salivary and serum cotinine in identifying tobacco-smoking status of self-reported non-smokers and smokers of tobacco and/or marijuana. Arch Environ Health, 44(1), 53-58.
Watts, R.R., Longone, J.J., Kinght, G.J., & Lewtas, J. (1990). Cotinine analytical workshop report: Consideration of analytical methods for determining cotinine in human body fluids as a measure of passive exposure to tobacco smoke. Environ Health Perspect, 84, 173-82.
Roche, D., Callai, F., Reungoat, P., & Momas, I. (2001). Adaptation of an enzyme immunoassay to assess urinary cotinine in nonsmokers exposed to tobacco smoke. Clin Chem, 47(5), 950-52.
Chard, T. (1990). An introduction to radioimmunoassay and related techniques (4th ed.). Amsterdam: Elsevier.
Granger, D.A., Blair, C., Willoughby, M., Kivlighan, K.T., Hibel, L.C., Fortunato, C.K., Wiegand, L.E., & The Family Life Project Investigators (2007). Individual differences in salivary cortisol and alpha-amylase in mothers and infants: Relation to tobacco smoke exposure. Dev Psychobiol, 49(7), 692-701.
Work one-on-one with Dr. Douglas Granger, a distinguished salivary bioscience researcher, to advance and refine your ideas, problem solve research design issues, pre-review proposal concepts, and rework plans for grant resubmissions.