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Diagnostic Salivary Cotinine ELISA Kit (FDA, CE Mark)

Technical Summary

Assay Protocol
Rev. 03.02.18
Catalog#: 1-2112
Regulatory Status: 510(k) Exempt, CE Mark
Format: 96-well plate
Assay Time: ~ 2.5 hrs
Sample Volume/Test: 20 µL
Sensitivity: 0.15 ng/mL
Assay Range: 0.8 ng/mL - 200 ng/mL
Storage Requirements: 2-8°C
Tests Per Kit
Singlet: 76
Duplicate: 38
Target Analyte

References & Salivary Cotinine Research

  1. Benowitz, N.L. (1996). Cotinine as a biomarker of environmental tobacco smoke exposure.  Epidemiol Rev, 18(2), 188-204.
  2. Dhar, P. (2004). Measuring tobacco smoke exposure: Quantifying nicotine/cotinine concentration in biological samples by colorimetry, chromatography and immunoassay methods. J Pharm Biomed Anal, 35(1), 155- 68.
  3. Alterman, A.I., Gariti, P., Niedbala, R.S. (2002). Varying results for immunoassay screening kits for cotinine levels. Psychol Addict Behav, 16(3), 256-59.
  4. Van Vunakis, H., Tashkin, D.P., Rigas, B., et al. (1989). Relative sensitivity and specificity of salivary and serum cotinine in identifying tobacco-smoking status of self-reported non-smokers and smokers of tobacco and/or marijuana. Arch Environ  Health, 44(1), 53-58.
  5. Benowitz, N.L., Hukkanen, J., & Jacob, P., 3rd (2009). Nicotine chemistry, metabolism, kinetics and biomarkers.  Handb Exp Pharmacol, 192, 29-60.
  6. Watts, R.R., Longone, J.J., Kinght, G.J., & Lewtas, J. (1990). Cotinine analytical workshop report: Consideration of analytical methods for determining cotinine in human body fluids as a measure of passive exposure to tobacco smoke.  Environ Health Perspect, 84, 173-82.
  7. Roche, D., Callai, F., Reungoat, P., & Momas, I. (2001). Adaptation of an enzyme immunoassay to assess urinary cotinine in nonsmokers exposed to tobacco smoke.  Clin Chem, 47(5), 950-52.
  8. Chard, T. (1990). An introduction to radioimmunoassay and related techniques (4th ed.).  Amsterdam: Elsevier.
  9. Granger, D.A., Blair, C., Willoughby, M., Kivlighan, K.T., Hibel, L.C., Fortunato, C.K., Wiegand, L.E., & The Family Life Project Investigators (2007). Individual differences in salivary cortisol and alpha-amylase in mothers and infants: Relation to tobacco smoke exposure.  Dev Psychobiol, 49(7), 692-701.
Contact: Salimetrics (USA)
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Salimetrics’ COVID-19 Status – 8 April 2020 – No change to Prior Update:

Salimetrics’ Sales and Customer Service, as well as all other non-laboratory personnel, have successfully transitioned to working remote and are fully able to provide support to our customers.

Our Salimetrics Pennsylvania Manufacturing Center remains open for customer orders and shipments. All Salimetrics’ laboratory personnel are following PPE guidelines per CDC Biosafety Level 2 (BSL-2).

The Salimetrics’ SalivaLab, located in California, is open to receive samples. In accordance with State of California Executive Order N-33-20 effective March 19, 2020, our Carlsbad, California facility, including R&D and SalivaLab personnel, will remain open working on research critical to the COVID-19 response; onsite SalivaLab personnel will be able to receive samples, and in some cases, test and send data.  All Salimetrics’ laboratory personnel are following PPE guidelines per CDC Biosafety Level 2 (BSL-2).



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