In 2009, two large genome wide association studies about body mass index (BMI) were conducted that further confirmed variants in the FTO gene that are linked to obesity (4, 5). More recently, Claussnitzer et al 2015, showed that the single T-to-C variant in rs1421085 functionally led to a reduction in mitochondrial thermogenesis in adipocyte precursor cells, and an increase in lipid storage, resulting in a direct effect on obesity (6).
Another SNP in the FTO gene, rs1121980, showed a strong association with early onset obesity, and how its effects could be attenuated with physical activity (7, 8). Closer evaluation of the SNPs within the FTO gene can aid researchers in discovering how genetic factors influence an outcome of obesity. For more research and details on MR visit SNPedia here.
When interpreting data from your current study or planning for the future, consider the polymorphisms that influence the regulation of body weight. For a complete list of SNPs available for research, we recommend contacting one of our experts for guidance on the most relevant selections for your research.
|Gene:||Fat Mass and Obesity|
|Related Analyte(s):||Insulin, Uric Acid, Cortisol|
|Genomic DNA, purified using silica based membrane columns is analyzed by TaqMan® SNP Genotyping Assays run on the 7900HT real time PCR system
Yield: 2-5 ug genomic DNA
Collect Saliva Samples
DNA SALIVA COLLECTION CONSIDERATIONS
Better results begin with better saliva collection. This collection protocol features general considerations to maximize salivary DNA analysis. Use this collection protocol to plan your collection methodology and sampling schemes.
APPROVED SALIVARY DNA COLLECTION METHODS
Test Saliva Samples
|Order Code (lab):||5811 (SNP Genotyping)|
|Transport Requirements:||Ship on Dry Ice|
The validated method used by Salimetrics is proprietary and not available in assay kit form at this time.
References & FTO Related Research
- Loos, R. J. F., & Yeo, G. S. H. (2014). The bigger picture of FTO – the first GWAS-identified obesity gene. Nature Reviews. Endocrinology, 10(1), 51–61.
- Scuteri, A., et al. (2007). Genome-Wide Association Scan Shows Genetic Variants in the FTO Gene Are Associated with Obesity-Related Traits. PLoS Genetics, 3(7), e115.
- Saldaña-Alvarez, Y., et al. (2016). Gender-Dependent Association of FTO Polymorphisms with Body Mass Index in Mexicans. PLoS ONE, 11(1), e0145984.
- Willer, C. J., et al. (2009). Six new loci associated with body mass index highlight a neuronal influence on body weight regulation. Nature Genetics, 41(1), 25–34.
- Thorleifsson, G., et al. (2008). Genome-wide association yields new sequence variants at seven loci that associate with measures of obesity. Nature Genetics, 41(1): 18–24.
- Claussnitzer, M., et al. (2015). FTO Obesity Variant Circuitry and Adipocyte Browning in Humans. The New England Journal of Medicine, 373(10): 895–907.
- Hinney, A., et al. (2007). Genome Wide Association (GWA) Study for Early Onset Extreme Obesity Supports the Role of Fat Mass and Obesity Associated Gene (FTO) Variants. PLoS ONE, 2(12), e1361.
- Karani S., et al (2009). Physical activity attenuates the body mass index-increasing influence of genetic variation in the FTO gene. The American Journal of Clinical Nutrition, 90(2): 425–428.