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Diagnostic Salivary DHEA ELISA Kit (CE Mark)

Technical Summary

Assay Protocol
Rev. 03.02.18
Specifications
Catalog#: 1-2212
Regulatory Status: CE Mark
Format: 96-well plate
Assay Time: ~ 4 hrs
Sample Volume/Test: 50 µL
Sensitivity: 5 pg/mL
Assay Range: 10.2 pg/mL - 1000 pg/mL
Storage Requirements: 2-8°C
Tests Per Kit
Singlet: 76
Duplicate: 38
Target Analyte
Technical Documentation

References & Salivary DHEA Research

    1. Kroboth, P.D., Salek, F.S., Pittenger, A.L., et al. (1999).  DHEA and DHEA-S: A review.  J Clin Pharmacol, 39(4), 327-48.
    2. Labrie, F. (2010).  DHEA, important source of sex steroids in men and even more in women.  Prog Brain Res, 182, 97-148.
    3. Labrie, F., Bélanger, A., Cusan, L., Candas, B. (1997).  Physiological changes in dehydroepiandrosterone are not reflected by serum levels of active androgens and estrogens but of their metabolites: Intracrinology.  J Clin Endocrinol Metab, 82(8), 2403-9.
    4. Labrie, F., Luu-The, V. Bélanger, A., et al. (2005).  Is dehydroepiandrosterone a hormone? J Endocrinol, 187, 169-96.
    5. Maninger, N., Wolkowitz, O.M., Reus, V.I., et al. (2009). Neurobiological and neuropsychiatric effects of dehydroepiandrosterone (DHEA) and DHEA sulfate (DHEAS).  Front Neuroendocrinol, 30(1), 65-91.
    6. Dillon, J.S. (2005).  Dehydroepiandrosterone, dehydroepiandrosterone sulfate and related steroids: Their role in inflammatory, allergic and immunological disorders.  Curr Drug Targets Infl Allergy, 4(3), 377-85.
    7. Widstrom, R., Dillon, J.S. (2004).  Is there a receptor for dehydroepiandrosterone or dehydroepiandrosterone sulfate?  Semin Repro Med, 22(4), 289-98.
    8. Baulieu, E.-E., Robel, P. (1998). Dehydroepiandrosterone (DHEA) and dehydroepiandrosterone sulfate (DHEA) as neuroactive neurosteroids.  Proc Natl Acad Sci U S A, 95(8), 4089-91.
    9. Charalampopoulos, I., Alexaki, V.-I., Tsatsanis, C., et al. (2006). Neurosteroids as endogenous inhibitors of neuronal cell apoptosis in aging.  Ann N Y Acad Sci, 1088, 139-52.
    10. Izawa, S. Sugaya, N., Shirotsuki, K., et al. (2008).  Salivary dehydroepiandrosterone secretion in response to acute psychosocial stress and its correlations with biological and psychological changes.  Biol Psych, 79(3), 294-98.

    1. Goodyer, I.M., Herbert, J., Altham, P.M., et al. (1996). Adrenal secretion during major depression in 8- to 16-year-olds, I. Altered diurnal rhythms in salivary cortisol and dehydroepiandrosterone (DHEA) at presentation. Psychol Med, 26(2), 245-56.
    2. Young, A.H., Gallagher, P., Porter, R. (2002). Elevation of the cortisol-dehydroepiandrosterone ratio in drug-free depressed patients.  Am J Psychiatry, 159(7), 1237-39.
    3. Harris, D.S., Wolkowitz, O.M., Reus, V.I. (2001). Movement disorder, memory, psychiatric symptoms and serum DHEA levels in schizophrienic and schizoaffective patients.  World J Biol Psychiatry, 2(2), 99-102.
    4. Christeff, N., Gherbi, N., Mammes, O., et al. (1997). Serum cortisol and DHEA concentrations during HIV infection.  Psychoneuroendocrinology, 22 (Suppl. 1), S11-18.
    5. Rosenfeld, R.S., Rosenberg, B.J., Fukushima, D.K., Hellman, L. (1975).  24-Hour secretory pattern of dehydroisoandrosterone and dehydroisoandrosterone sulfate.       J Clin Endocrinol Metab, 40(5), 850-5.
    6. Longcope, C. (1995). Metabolism of dehydroepiandrosterone.  Ann N Y Acad Sci, 774, 143-48.
    7. Simon, J.A. (2002). Estrogen replacement therapy: Effect on the endogenous androgen milieu.  Fertil Steril, 77 (Suppl 4), S77-82.)
    8. Vining, R.F., McGinley, R.A., Symons, R.G. (1983). Hormones in saliva: Mode of entry and consequent implications for clinical interpretation.  Clin Chem, 29(10), 1752-56.
    9. Shirtcliff, E.A., Granger, D.A., Schwartz, E., Curran, M.J. (2001). Use of salivary biomarkers in biobehavioral research: Cotton-based sample collection methods can interfere with salivary immunoassay results.  Psychoneuroendocrinology, 26(2), 165-73.)

Contact: Salimetrics (USA)
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Salimetrics’ COVID-19 Status – 6 April 2020 – No change to Prior Update:

Salimetrics’ Sales and Customer Service, as well as all other non-laboratory personnel, have successfully transitioned to working remote and are fully able to provide support to our customers.

Our Salimetrics Pennsylvania Manufacturing Center remains open for customer orders and shipments. All Salimetrics’ laboratory personnel are following PPE guidelines per CDC Biosafety Level 2 (BSL-2).

The Salimetrics’ SalivaLab, located in California, is open to receive samples. In accordance with State of California Executive Order N-33-20 effective March 19, 2020, our Carlsbad, California facility, including R&D and SalivaLab personnel, will remain open working on research critical to the COVID-19 response; onsite SalivaLab personnel will be able to receive samples, and in some cases, test and send data.  All Salimetrics’ laboratory personnel are following PPE guidelines per CDC Biosafety Level 2 (BSL-2).

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