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Diagnostic Salivary Cortisol ELISA Kit (FDA, CE Mark)

Biomarkers

Technical Summary

Assay Protocol
Rev. 09.09.21 (1-3102)

Rev. 09.06.24 (1-3102-b)
Specifications
Catalog#: 1-3102
Regulatory Status: 510(k), CE Mark
Format: 96-well plate
Assay Time: ~ 2 hrs
Sample Volume/Test: 25 µL
Sensitivity: <0.007 ug/dL
Assay Range: 0.012-3.000 ug/dL
Storage Requirements: 2-8°C
Tests Per Kit
Singlet: 76
Duplicate: 38
Target Analyte
Technical Documentation

References & Salivary Cortisol Research

    1. Migeon, C.J., & Lanes, R.L. (1990). Adrenal cortex: hypo- and hyperfunction. In F. Lifshitz (ed.), Pediatric endocrinology, a clinical guide (2nd ed.), (pp. 333-52). New York: Marcel Dekker.
    2. Drucker, S., New, M.I. (1987).  Disorders of adrenal steroidogenesis. Pediatr Clin North Am, 34(4), 1055-66.
    3. Fischbach, F.T. (1992). The manual of laboratory and diagnostic tests, (4th ed.). Philadelphia: J. B. Lippincott.
    4. Dorn, L.D., Lucke, J.F., Loucks, T.L., Berga, S.L. (2007). Salivary cortisol reflects serum cortisol: analysis of circadian profiles. Ann Clin Biochem, 44(pt 3), 281-84.
    5. Chernow, B., Alexander, H.R., Smallridge, R.C., et al. (1987). Hormonal responses to graded surgical stress. Arch Intern Med, 147(7), 1273-78.
    6. Kreiger, D.T. (1975). Rhythms of ACTH and corticosteroid secretion in health and disease and their experimental modification. J Steroid Biochem, 6(5), 758-91.
    7. Rothfield, B. (1974). Plasma cortisol. In: B. Rothfield (ed.), Nuclear medicine–in vitro (pp. 120-5). Philadelphia: Lippincott.
    8. Robin, P., Predine, J., Milgrom, E. (1977). Assay of unbound cortisol in plasma. J Clin Endocrinol Metab, 46(2), 277-83.
    9. Vining, R.F., McGinley, R.A., Symons, R.G. (1983). Hormones in saliva: mode of entry and consequent implications for clinical interpretation. Clin Chem, 29(10), 1752-56.
    10. Vining, R.F., McGinley, R.A. (1987). The measurement of hormones in saliva: Possibilities and pitfalls. J Steroid Biochem, 27(1-3), 81-94.

  1. Francis, S.J., Walker, R.F., Riad-Fahmy, D., et al. (1987). Assessment of adrenocortical activity in term newborn infants using salivary cortisol determinations. J of Pediatrics, 111, 129-33.
  2. Hiramatsu, R. (1981). Direct assay of cortisol in human saliva by solid phase radioimmunoassay and its clinical applications. Clinica Chimica Acta, 117, 239-249.
  3. Vining, R.F., McGinley, R.A., Maksvytis, J.J., Ho, K.Y. (1983). Salivary cortisol: A better measure of adrenal cortical function than serum cortisol. Ann Clin Biochem, 20(pt 6), 329-35.

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