Salimetrics Assay #1-3702The Salimetrics Salivary Estradiol (17-beta estradiol, oestradiol) Enzyme Immunoassay Kit was specifically designed to standardize the detection of estradiol in saliva samples for research and biomedical laboratories. Using a small sample volume, this assay kit has an extended range that spans the expected estradiol levels found in human saliva. The average inter- and intra-assay precision coefficients of variation are low with no deleterious matrix effects often found in saliva which are characterized through dilution- and spike-recovery validation procedures. This estradiol assay kit has also been formatted to minimize cross reactivity for related steroids.Estradiol (17β-estradiol, E2, 1,3,5(10)-estratriene-3, 17β-diol) is the most active naturally secreted estrogen. In men, estradiol originates in the testes and from extraglandular conversion of androgens. Circulating estradiol levels are relatively high at birth in both males and females, but decrease postnatally. Research concerning estradiol has focused predominantly on reproductive issues such as conception, ovulation, infertility, and menopause. Yet, estradiol affects a diversity of biological processes involved with pubertal and reproductive capacity, establishment and maintenance of pregnancy, infant care, coronary artery disease, immunocompetence, and cancer susceptibility. Estradiol is also believed to affect individual differences in cognitive and socioemotional processes, as well as psychopathology.
|Assay Time:||~ 3 hrs|
|Sample Volume/Test:||100 µL|
|Assay Range:||1 pg/mL - 32 pg/mL|
Salivary Estradiol Assay Kit Overview
The Salimetrics 17β-Estradiol Enzyme Immunoassay Kit is a competitive immunoassay specifically designed and validated for the quantitative measurement of salivary Estradiol. It is not intended for diagnostic use. It is intended only for research use in humans and some animals. Salimetrics has not validated this kit for serum or plasma samples.
Estradiol (17β-Estradiol, E2, 1,3,5(10)-estratriene-3, 17β-diol), a steroid hormone, is produced primarily by the ovarian follicles from testosterone. Estradiol is the most active naturally secreted estrogen. In men, Estradiol originates in the testes and from extraglandular conversion of androgens. Circulating Estradiol levels are relatively high at birth in both males and females, but decrease postnatally. In prepubertal children and men, levels are non-cyclic and low. During puberty, there are gradual increases in Estradiol levels in both males and females. Interactions between luteinizing hormone (LH) and follicle-stimulating hormone (FSH) cause the release of Estradiol from the ovaries in premenopausal women. Estradiol secretion is low in postmenopausal women. Research concerning Estradiol has focused predominantly on reproductive issues such as conception, ovulation, infertility, and menopause. Yet, Estradiol affects a diversity of biological processes involved with reproductive capacity, establishment and maintenance of pregnancy, parenting, coronary artery disease, immunocompetence, cancer susceptibility, and neuroprotection. Estradiol is also believed to affect individual differences in cognitive and socioemotional processes as well as psychopathology. Estrogens have been measured by many immunoassay methods. Studies suggest that Estradiol can be accurately measured in saliva.
Salivary Estradiol Assay Principle
This is a competitive immunoassay kit. Estradiol in standards and samples compete with Estradiol conjugated to horseradish peroxidase for the antibody binding sites on a microtitre plate. After incubation, unbound components are washed away. Bound Estradiol Enzyme Conjugate is measured by the reaction of the horseradish peroxidase enzyme to the substrate tetramethylbenzidine (TMB). This reaction produces a blue color. A yellow color is formed after stopping the reaction with an acidic solution. The optical density is read on a standard plate reader at 450 nm. The amount of Estradiol Enzyme Conjugate detected is inversely proportional to the amount of Estradiol present in the sample.
References & Salivary Estradiol Research
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- Choe, J.K., Khan-Dawood, F.S., Dawood, M.Y. (1982). Progesterone and estradiol in saliva and plasma during the menstrual cycle. Am J Obstet Gynecol, 146, 557-62.
- Bao, A.-M., Liu, R.-Y., van Someren, E.J., et al. (2003). Diurnal rhythm of free estradiol during the menstrual cycle. Eur J Endocrinol, 148(2), 227-32.
- Chang, R.J., Plouffe Jr., L. Schaffer, K. Physiology of the menopause. In: Comprehensive management of menopause, Lorrain, J., Flouffe Jr., L., Ravnikar, V., Speroff, L., Watts, N., eds. New York: Springer, 1993.
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- Winters, S.J., Troen, P. (1986). Testosterone and estradiol are co-secreted episodically by the human testis. J Clin Invest, 78(4), 870-73.
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- Balthazart, J., Cornil, C.A., Taziaux, M., et al. (2006). Rapid changes in production and behavioral action of estrogens. Neuroscience, 138(3), 783-91.
- Karpuzoglu, E., Ahmed, S.A. (2006). Estrogen regulation of nitric oxide and inducible nitric oxide synthase (iNOS) in immune cells: Implications for immunity, autoimmune diseases, and apoptosis. Nitric Oxide, 15(3), 177-86.
- Colditz, G. A. 1998. Relationship between estrogen levels, use of hormone replacement therapy, and breast cancer. J Natl Cancer Inst, 90(11), 814-23.
- Lépine, J., Audet-Walsh, E., Grégoire, J., et al. (2010) Circulating estrogens in endometrial cancer cases and their relationship with tissular expression of key estrogen biosynthesis and metabolic pathways. J Clin Endocinol Metab, 95(6), 2689-98.
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- Vining, R.F., & McGinley, R.A. (1987). The measurement of hormones in saliva: Possibilities and pitfalls. J Steroid Biochem, 27(1-3), 81-94.
- Choe, J.K., Khan-Dawood, F.S., & Dawood, M.Y. (1983). Progesterone and estradiol in saliva and plasma during the menstrual cycle. Am J Obstet Gynecol, 147(5), 557-62.
- Shirtcliff, E.A., Granger, D.A., Schwartz, E.B., et al. (2000). Assessing estradiol in biobehavioral studies using saliva and blood spots: Simple radioimmunoassay protocols, reliability, and comparative validity. Horm Behav, 38(2), 137-47.
- Ellison, P.T., Lipson, S.F. (1999). Salivary estradiol–A viable alternative? Fertil Steril, 72(5), 951-52.)