Diagnostic Salivary DHEA ELISA Kit (CE Mark)

Salimetrics Assay #1-2212 (in vitro diagnostic use)

The Salimetrics Salivary DHEA Enzyme Immunoassay Kit was specifically designed to standardize the detection of DHEA in saliva samples for research and biomedical laboratories. Using a small sample volume, this assay kit has an extended range that spans the expected DHEA levels found in human saliva. The average inter- and intra-assay precision coefficients of variation are low with no deleterious matrix effects often found in saliva which are characterized through dilution- and spike-recovery validation procedures. This DHEA assay kit has also been formatted to minimize cross reactivity for related steroids. DHEA is a major secretory product of the adrenal glands; it is co-released along with cortisol in response to ACTH from the pituitary gland. A major role of DHEA is to act as a circulating precursor of androgens and estrogens in tissues throughout the body, and it has also been associated with immune function. In addition, DHEA is produced directly by the nervous system, where it functions as a neuroactive and neuroprotective factor. Salivary DHEA levels are unaffected by salivary flow rate or salivary enzymes. DHEA exhibits a diurnal rhythm similar to cortisol, with highest levels in the morning after awakening, followed by a decline throughout the afternoon and evening.

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Technical Summary

Assay Protocol
Download Rev. 09.27.24

Specifications
Catalog#:	1-2212
Regulatory Status:	CE Mark
Format:	96-well plate
Assay Time:	~ 4 hrs
Sample Volume/Test:	50 µL
Sensitivity:	5 pg/mL
Assay Range:	10.2 pg/mL - 1000 pg/mL
Storage Requirements:	2-8°C

Tests Per Kit
Singlet:	76
Duplicate:	38

Target Analyte
Salivary DHEA

Technical Documentation
Stop Solution SDS

References & Salivary DHEA Research

1. Kroboth, P.D., Salek, F.S., Pittenger, A.L., et al. (1999). DHEA and DHEA-S: A review. J Clin Pharmacol, 39(4), 327-48.
2. Labrie, F. (2010). DHEA, important source of sex steroids in men and even more in women. Prog Brain Res, 182, 97-148.
3. Labrie, F., Bélanger, A., Cusan, L., Candas, B. (1997). Physiological changes in dehydroepiandrosterone are not reflected by serum levels of active androgens and estrogens but of their metabolites: Intracrinology. J Clin Endocrinol Metab, 82(8), 2403-9.
4. Labrie, F., Luu-The, V. Bélanger, A., et al. (2005). Is dehydroepiandrosterone a hormone? J Endocrinol, 187, 169-96.
5. Maninger, N., Wolkowitz, O.M., Reus, V.I., et al. (2009). Neurobiological and neuropsychiatric effects of dehydroepiandrosterone (DHEA) and DHEA sulfate (DHEAS). Front Neuroendocrinol, 30(1), 65-91.
6. Dillon, J.S. (2005). Dehydroepiandrosterone, dehydroepiandrosterone sulfate and related steroids: Their role in inflammatory, allergic and immunological disorders. Curr Drug Targets Infl Allergy, 4(3), 377-85.
7. Widstrom, R., Dillon, J.S. (2004). Is there a receptor for dehydroepiandrosterone or dehydroepiandrosterone sulfate? Semin Repro Med, 22(4), 289-98.
8. Baulieu, E.-E., Robel, P. (1998). Dehydroepiandrosterone (DHEA) and dehydroepiandrosterone sulfate (DHEA) as neuroactive neurosteroids. Proc Natl Acad Sci U S A, 95(8), 4089-91.
9. Charalampopoulos, I., Alexaki, V.-I., Tsatsanis, C., et al. (2006). Neurosteroids as endogenous inhibitors of neuronal cell apoptosis in aging. Ann N Y Acad Sci, 1088, 139-52.
10. Izawa, S. Sugaya, N., Shirotsuki, K., et al. (2008). Salivary dehydroepiandrosterone secretion in response to acute psychosocial stress and its correlations with biological and psychological changes. Biol Psych, 79(3), 294-98.

1. Goodyer, I.M., Herbert, J., Altham, P.M., et al. (1996). Adrenal secretion during major depression in 8- to 16-year-olds, I. Altered diurnal rhythms in salivary cortisol and dehydroepiandrosterone (DHEA) at presentation. Psychol Med, 26(2), 245-56.
2. Young, A.H., Gallagher, P., Porter, R. (2002). Elevation of the cortisol-dehydroepiandrosterone ratio in drug-free depressed patients. Am J Psychiatry, 159(7), 1237-39.
3. Harris, D.S., Wolkowitz, O.M., Reus, V.I. (2001). Movement disorder, memory, psychiatric symptoms and serum DHEA levels in schizophrienic and schizoaffective patients. World J Biol Psychiatry, 2(2), 99-102.
4. Christeff, N., Gherbi, N., Mammes, O., et al. (1997). Serum cortisol and DHEA concentrations during HIV infection. Psychoneuroendocrinology, 22 (Suppl. 1), S11-18.
5. Rosenfeld, R.S., Rosenberg, B.J., Fukushima, D.K., Hellman, L. (1975). 24-Hour secretory pattern of dehydroisoandrosterone and dehydroisoandrosterone sulfate. J Clin Endocrinol Metab, 40(5), 850-5.
6. Longcope, C. (1995). Metabolism of dehydroepiandrosterone. Ann N Y Acad Sci, 774, 143-48.
7. Simon, J.A. (2002). Estrogen replacement therapy: Effect on the endogenous androgen milieu. Fertil Steril, 77 (Suppl 4), S77-82.)
8. Vining, R.F., McGinley, R.A., Symons, R.G. (1983). Hormones in saliva: Mode of entry and consequent implications for clinical interpretation. Clin Chem, 29(10), 1752-56.
9. Shirtcliff, E.A., Granger, D.A., Schwartz, E., Curran, M.J. (2001). Use of salivary biomarkers in biobehavioral research: Cotton-based sample collection methods can interfere with salivary immunoassay results. Psychoneuroendocrinology, 26(2), 165-73.)

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Diagnostic Salivary DHEA ELISA Kit (CE Mark)

Salimetrics Assay #1-2212 (in vitro diagnostic use)

Technical Summary

References & Salivary DHEA Research

How Can we help?

Test Your DBS samples with Salimetrics!